This study was carried out to compare the efficacy of Рseudomonas putida, contained in the biopreparation «Pseudomin» in the bioremediation of diesel fuel contaminated derno-podzoluivisolic soil of two different horizons. By analyzing the Total Petroleum Hydrocarbons (TPH) content using IR-spectrometry method under laboratory conditions, results of the research showed that the inoculation of Рseudomonas putida in to the diesel fuel contaminated derno-podzoluivisolic soil samples in only 12 weeks was able to reduce the diesel fuel content up to 99.2% in the humus-accumulative horizon; and up to 92.8% in the eluvial horizon. Comparative evaluation of the contaminated variants inoculated with Pseudomonas of both horizons revealed a significantly higher rate of diesel fuel degradation in the humus accumulative horizon of up to 8.7 times, compared to the eluvial horizon, which shows a slightly slower rate of degradation.

This study examined the phytotoxic impact of transmission oil pollution on Oat (Aveena sativa) seedlings growing in contaminated soils under 3 different soil moisture conditions of 100% water saturation, 60% and 30% water contents. The synergistic role of the microorganism Pseudomonas putida as a bioremediating agent was also examined.Experimental soil was treated with transmission oil for up to 12 weeks but seedlings were grown and measured on weeks 1, 7 and 12 using derno-podzoluivisolic soil collected from forest reserve of the Russian State Agrarian University. Results showed that seedlings grown on week 7 and 12 germinated better but had variable growth rates. In addition, Pseudomonas putida seemed to improve seedling germination at lower water contents for both weeks. This experiment suggests that Pseudomonas putida may be a good candidate for bioremediation.

ABSTRACT:Analyzing the prospects of bioremediation methods and technologies as a potential clean up solution and remedy to the current environmental pollution problems facing the world today. Due to the significant rise in the number of manufacturing/ heavy industries, increase in the volume of crude oil drilling, and refining and transportation the area of contaminated land and water by heavy metals and petroleum hydrocarbon has consequently increased has a result of accidents, spills, dumping, etc., posing serious risk to human health and livelihood, as well disrupting the ecosystem and climate.Bioremediation methods and technologies can mostly be combined and carried out in a multi stage process to get best results. For instance, in the event of an oil spill the initial Raskin, Ensley, 2000;Tyagi, et al, 2010;, as opposed to previous harmful and environmentally degrading clean up procedure should involve the physical / mechanical removal of excess crude oil from the surface of the contaminated medium, by the use of hydraulic pumps, skimmers, boomers, surfactant's etc. At the second stage a suitable technique of microbial bioremediation can be applied, depending on the prevailing conditions. Lastly phytoremediation techniques can be applied at the final stages to complete the cleanup and restoration of the contaminated soil or water medium.