Aim: This study was conducted to determine the occurrence, antibiotic susceptibility pattern and physiological properties of Pseudomonas species isolated from ready to eat foods in some selected areas in Ibadan, Oyo state, Nigeria. Study Design: Identification of Pseudomonas species in ready to eat foods, determination of its antibiotics sensitivity pattern and physiological properties. Place and Duration of Study: All works were carried out in the Department of Microbiology,

This present work was designed to investigate the effect of different extraction solvents on the antimicrobial activity of Psidium guajava leaves against some multidrug resistant bacteria in nosocomial infections. The MDR isolates such as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, Proteus mirabilis, Staphylococcus saprophyticus and Bacillus cereus were obtained from Microbiology department culture collection of Department of Microbiology University of Ibadan Nigeria and their identities reconfirmed using biochemical methods. The antimicrobial activities of the different solvent extracts were tested using agar well diffusion method while the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the extracts were monitored using the double fold dilution method. The results reconfirmed the identities of the bacteria as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, Proteus mirabilis, Staphylococcus saprophyticus and Bacillus cereus. Methanol extracts at a concentration of 160 mg/ml showed the highest inhibition zone of 10.0±0.1mm followed by ethyl acetate extract with 8.0±0.6mm inhibition zone against E.coli which were significantly different (P<0.05) while Nhexane, cold and hot water showed zero inhibition zones. Ofloxacin and DMSO. showed 20.0±0.and 0.00 mm inhibition zones respectively. This trend was observed at all the other concentrations. Methanol and ethyl acetate had MIC of 40.0 mg/ml for E. coli, S. saprophyticus, P.aeruginosa and B. cereus and they showed MBC of 80.0mg/ml for E. coli, S. saprophyticus, P.aeruginosa and B. cereus and MBC of 40.0 mg/ml for S. Epidermidis. Methanol and ethyl acetate extracts could be employed in the treatment of bacterial infections caused by MDR bacteria.Contribution/Originality: The paper's primary contribution is finding that Psidium guajava (guava) leaf extracts can be used for the treatment of some multi-drug resistant bacteria implicated in nosocomial infections.Methanol and ethyl acetate extracts of the leaves possess the greatest antimicrobial activity due to the high sensitivity of the MDR organisms.

No abstract

Aim: To investigate the production characteristics and molecular properties of protease from Pediococcus acidilactici under cold storage temperature. Study Design: Identification and re-identification of Pediococcus acidilactis and determination of optimal conditions for protease production and molecular properties.

Aim: To optimize lipase production by Bacillus megaterium in submerged fermentation. Study Design: Collection of palm oil press fibres and effluent from different palm oil mills located within Ibadan Municipality. Isolation of Bacillus megaterium by cultivation in medium, submerged fermentation of palm oil press fibres and effluent by B. megaterium to produce lipase. Alteration of the cultural conditions to optimize production. Place and Duration of Study: All work were done in the Methodology: Palm oil press fibres and effluent were collected from various palm oil mills and were used as the source of isolation of microorganism. The isolated species were identified by studying the morphological, biochemical, characteristics and 16SrNA gene sequencing. The selected species was screened for lipase production. Results:The results obtained revealed that maximum lipase production was recorded at pH 7.0 with an activity 2.13±0.15 U/ml while the best temperature that supported the optimum production of 2 lipase was seen at 35°C with an activity of 3.30±0.10 U/ml and the best carbon and nitrogen sources were 2% glucose and 2.5% peptone concentrations showing activities of 1.83±0.05 U/ml and 2.60±0.10 U/ml respectively. An incubation period of 72 hours produced the optimum lipase with an activity of 3.26±0.05 U/ml. The separate additions of 0.3M Ca 2+ and 0.3M Clsupported maximum production of lipase. Conclusion: This study showed that lipase production by B. megaterium can be optimized and the best conditions for optimization included pH 7.0, temperature of 35°C, 72 hours incubation period in the presence of 2% glucose, 2.5% peptone concentrations and 0.3M Ca 2+ and 0.3M Cl -.