Importantly to study the coastal vegetation from Kekara Laut (Canavalia maritima Thou.) to observe the antibacterial agents towards Staphylococcus epidermidis. The purpose of this study to examine the variation concentration effect of an ethanol leaf extract from Canavalia maritima Thou. in inhibiting the growth of S. epiderimidis bacteria. The leaf of Canavalia maritima Thou. was prepared using a physicochemical method to obtain ethanol extract, then varied in several concentrations, namely 5% w/v; 10% w/v; 15% w/v; and 20% w/v. Based on these results, we obtain the ethanol leaf extract can inhibit the growth of S. epidermidis with a concentration of 15% w/v for 24 hours having an inhibition zone of 17.17 mm. The statistical analysis test was very significantly different from all antibacterial tests, the value of F count was 682.1 with F table (3.48), at the level of α = 0.05 (3.48) and α = 0.01 (5.99), which indicates that H0 is rejected, but using the Variance method in the Newman-Keuls range approach shows that each concentration has a good inhibitory ability. The use of Canavalia maritima Thou. leaf extract in low concentrations can significantly inhibit bacterial growth (bacteriostatic).

Quality drinking water was assessed from microbiological parameters, is the absence of total coliform and Escherichia coli bacteria in 100 ml samples. The purpose was to identify Escherichia coli bacteria in refill drinking water produced by refill drinking water depot at Perumnas IV Waena Abepura. The research was conducted at the Laboratory of Microbiology, Health Polytechnic of the Ministry of Health in Jayapura. Water samples obtained from 6 depots. This type of research is descriptive. The quality of water bacteriology uses the Most Probable Number (MPN) method which consists of a presumptive test using 3 3 3 tube Lactose Broth (LB) media, and a Confirmation Test using Brillian Green Lactose Broth (BGLB) media, complete test using EMB agar. The results showed that at 6 water depots there were 3 negative samples or no Escherichia coli bacteria were found and 3 positive samples found Escherichia coli bacteria.

Areca nut (Areca catechu Linn) is a plant that is widely distributed in tropical and subtropical regions. People have used it to treat various health problems. This study aims to determine the ability of betel nut ethanol extract to inhibit the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is experimental. Stages of research, first the betel nut is dried by aerating for 7-10 days. After drying, the seeds are ground into powder. The extraction stage of the active compounds uses the maceration method using 92% ethanol, evaporation is carried out using a rotary vacuum evaporator. The results of the maceration extract were carried out with phytochemical tests and bacterial inhibitory tests against E. coli, and S. aureus, carried out by the Kirby Bauer method. The detected metabolites were flavonoids, tannins and terpenoids. These metabolites have the ability as antimicrobials. The results of the inhibition test of the ethanol extract of areca nut seeds showed descriptively that the ethanol extract had a better inhibitory ability against S. aureus bacteria, compared to E. coli. The conclusion of this study is that the ethanol extract of areca nut seeds can inhibit the growth of E.coli and S.aureus.

Kualitas udara dalam ruangan (Indoor Air Quality) merupakan masalah yang perlu mendapat perhatian karena akan berpengaruh terhadap kesehatan manusia. Bakteri yang terdapat pada udara ruangan rumah sakit merupakan salah satu penyebab penyakit infeksi yang disebut infeksi nosokomial. Penelitian ini merupakan penelitian deskriptif dengan pendekatan observasional laboratorium untuk mengetahui kualitas bakteri udara di ruang operasi Rumah Sakit Abepura dan Rumah Sakit Dian Harapan di kota Jayapura. Penentuan jumlah sampel yang digunakan pada penelitian ini menggunakan metode total sampling, yaitu mengambil seluruh sampel ruang operasi di Rumah Sakit Umum Daerah (RSUD) Abepura dan Rumah Sakit Dian Harapan di Kota Jayapura. Setiap ruang diambil satu kali yaitu pada pagi hari dengan 2 titik pengambilan sampel. Tahapan penelitian ini di mulai dengan meletakan cawan petri yang berisi media PCA dan menghitung jumlah total angka kuman diruang operasi Rumah Sakit Abepura dan RS Dian Harapan. Hasil penelitian di RS Abepura indeks angka kuman pada ruang OK 1 sebesar 13 CFU/M³, OK2 sebesar 4 CFU/M³, OK 3 sebesar 1,3 CFU/M³ dan ruang OK 4 sebesar 0 CFU/M3. Hasil penelitian di RS Dian Harapan indeks angka kuman pada ruang OK 1 sebesar 4 CFU/M³, OK2 = 0 CFU/M3, OK 3 sebesar 6,5 CFU/M³ dan ruang OK 4 sebesar 11,7 CFU//M3. Kesimpulannya bahwa semua ruang operasi di RSUD Abepura dan RS Dian Harapan masih memenuhi syarat sesuai dengan baku mutu Permenkes no. 7 tahun 2019.

Chlorine is a chemical that functions as water disinfection, often used in swimming pools to kill microorganisms such as pathogenic bacteria in the water. The general objective of this study was to determine the relationship between chlorine levels, and microbiological quality in swimming pool water in Jayapura City. This type of research is descriptive research with a cross-sectional design. Determination of residual chlorine content, and microbiological quality in this study was carried out using Spectrophotometry, and MPN Test methods. The results showed that the chlorine content of the swimming pool Wa 5.00 mg/l, Fu 0.23 mg/l, and Aj 0.13 mg/l. The examination of the coliform bacteria population showed that the MPN value in Wa coliform swimming pool water was 0/100 ml, and coliform stools were 0/100 ml. Swimming pool Fu coliform as much as 91/100 ml, and fecal coliform as much as  23 /100 ml. Swimming pool Aj coliform as much as >1100/100 ml, and fecal coliform as 20/100 ml. This study concludes that the chlorine levels in the Wa swimming pools, and the MPN swimming pools of Fu, and Aj are not under the Minister of Health Regulation No. 32 years 2017. Keywords : Coliform, Swimming pool, MPN