Kefir starter culture was used for the production of beverages, and some chemical and microbiological parameter changes were determined during 24 h of fermentation and then after 2, 7, 14, 21 and 28 days of storage at 4°C. Three different substrates (milk, whey and soya milk) were used as fermentation media for the kefir starter culture. After the fermentation, the carbohydrate content and pH decreased, but the dry matter and fat content of the beverages were not significantly different from their substrates. During storage, lactic acid bacteria in the beverages decreased, while yeasts increased and carbohydrate, ethanol and pH changed significantly in the three beverages.

If you would like to write for this, or any other Emerald publication, then please use our Emerald for Authors service information about how to choose which publication to write for and submission guidelines are available for all. Please visit www.emeraldinsight.com/authors for more information. About Emerald www.emeraldinsight.comEmerald is a global publisher linking research and practice to the benefit of society. The company manages a portfolio of more than 290 journals and over 2,350 books and book series volumes, as well as providing an extensive range of online products and additional customer resources and services.Emerald is both COUNTER 4 and TRANSFER compliant. The organization is a partner of the Committee on Publication Ethics (COPE) and also works with Portico and the LOCKSS initiative for digital archive preservation. AbstractPurpose -Milk whey is a by-product of the cheese-making industry which presents about 85-95 per cent of the milk volume. Whey fermentation by the isolated kefir starter culture could be a sensible solution for the use of this by-product from the dairy industry. For the first time, in Iran the microbial flora of kefir grain was isolated and identified (Motaghi et al.). This paper aims to examine the various ratios of starter culture of kefir grain for production of fermented beverage using whey as the substrate. Design/methodology/approach -Various ratios of lactic bacteria, yeasts and acetic acid bacteria were tested. The incubation time (24 h) temperature (25 C), substrate (pasteurized whey), and inoculation rate (3-5 per cent) and mixing rate (90 rpm) were the same for the all products. The products were analyzed for protein, fat, sugar, alcohol, carbon dioxide, acidity, density, dry material, ash, and riboflavin content.Findings -The quality (smell and flavor) of the product was assessed. Samples produced with 3 per cent (v/v) lactic acid, acetic acid bacterial mixed cultures and 2 per cent (v/v) yeast culture were considered as best with respect to quality and organoleptic quality. Originality/value -The ability of kefir starter cultures to ferment whey to formulate and mix cultures consisting of certain species of kefir grain micro-organisms in-order to develop a procedure where lactic acid fermentation is followed by yeast fermentation. The potential of local kefir starter culture in production of healthy beverage from whey is a new finding since this particular beverage has the taste of artificial butter milk and it is naturally carbonated.

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