In this study, we propose a novel method to predict the solvent accessible surface areas of transmembrane residues. For both transmembrane alpha-helix and beta-barrel residues, the correlation coefficients between the predicted and observed accessible surface areas are around 0.65. On the basis of predicted accessible surface areas, residues exposed to the lipid environment or buried inside a protein can be identified by using certain cutoff thresholds. We have extensively examined our approach based on different definitions of accessible surface areas and a variety of sets of control parameters. Given that experimentally determining the structures of membrane proteins is very difficult and membrane proteins are actually abundant in nature, our approach is useful for theoretically modeling membrane protein tertiary structures, particularly for modeling the assembly of transmembrane domains. This approach can be used to annotate the membrane proteins in proteomes to provide extra structural and functional information.

We present here LOCATE, a curated, web-accessible database that houses data describing the membrane organization and subcellular localization of proteins from the FANTOM3 Isoform Protein Sequence set. Membrane organization is predicted by the highthroughput, computational pipeline MemO. The subcellular locations of selected proteins from this set were determined by a high-throughput, immunofluorescence-based assay and by manually reviewing .1700 peer-reviewed publications. LOCATE represents the first effort to catalogue the experimentally verified subcellular location and membrane organization of mammalian proteins using a high-throughput approach and provides localization data for 40% of the mouse proteome. It is available at http://locate. imb.uq.edu.au.

Membrane organization describes the orientation of a protein with respect to the membrane and can be determined by the presence, or absence, and organization within the protein sequence of two features: endoplasmic reticulum signal peptides and alpha-helical transmembrane domains. These features allow protein sequences to be classified into one of five membrane organization categories: soluble intracellular proteins, soluble secreted proteins, type I membrane proteins, type II membrane proteins, and multi-spanning membrane proteins. Generation of protein isoforms with variable membrane organizations can change a protein's subcellular localization or association with the membrane. Application of MemO, a membrane organization annotation pipeline, to the FANTOM3 Isoform Protein Sequence mouse protein set revealed that within the 8,032 transcriptional units (TUs) with multiple protein isoforms, 573 had variation in their use of signal peptides, 1,527 had variation in their use of transmembrane domains, and 615 generated protein isoforms from distinct membrane organization classes. The mechanisms underlying these transcript variations were analyzed. While TUs were identified encoding all pairwise combinations of membrane organization categories, the most common was conversion of membrane proteins to soluble proteins. Observed within our high-confidence set were 156 TUs predicted to generate both extracellular soluble and membrane proteins, and 217 TUs generating both intracellular soluble and membrane proteins. The differential use of endoplasmic reticulum signal peptides and transmembrane domains is a common occurrence within the variable protein output of TUs. The generation of protein isoforms that are targeted to multiple subcellular locations represents a major functional consequence of transcript variation within the mouse transcriptome.

h i g h l i g h t sFibers are added for enhancing the ultimate flexural capacity of reinforced SCC beam. Using steel fibers to partially replace conventional steel rebar is feasible. One large crack developed in pure bending section of SFRSCC beam after yield of steel rebar. The suggested model for estimating ultimate moment of SFRSCC beam agrees well with test data. a b s t r a c tSeven full-scale steel fiber reinforced self-consolidating concrete (SFRSCC) beams were tested to study the effects of macro steel fibers on the flexural behavior of reinforced self-consolidating concrete beams. The major test variables are fiber contents and longitudinal reinforcement ratios. The ultimate load, midspan deflections, steel reinforcement strains, crack width and crack spacing were investigated. The enhanced ultimate flexural capacity and reduced midspan deflection due to the addition of steel fibers were observed. With the increasing of fiber contents, the strain in longitudinal reinforcement, crack width and crack spacing decreased significantly. The possibility of using steel fibers for partial replacement of the conventional longitudinal reinforcement is estimated, which is meaningful for extending the structural application of SFRSCC. A method incorporating fiber contribution to the post-cracking tensile strength of concrete in the flexural analysis of SFRSCC beam is also suggested. Comparisons are made between the suggested model and the fib Model Code 2010 model with experimental data. The results showed that the suggested model can estimate ultimate flexural capacity accurately.

No abstract