Objectives: This study aimed to isolate the polyphenolic constituents from the methanol extract of Livistona decipiens Becc leaves and evaluate the antioxidant, cytotoxic and antimicrobial activities of the total methanol extract and ethyl acetate fraction. Methods: The ethyl acetate and n-butanol fractions of Livistona decipiens leaf methanol extract were subjected separately to different chromatographic separation techniques. Structures of the isolated compounds were established by different spectroscopic techniques (1 H / 13 C NMR). Antioxidant activity was evaluated by DPPH assay, while evaluation of cytotoxicity was done according to MTT cell viability assay. Antimicrobial activity was done by agar diffusion method. Results: seven compounds were isolated from the ethyl acetate and n-butanol fractions, five compounds were identified for the first time from this plant as apigenin-8-C-β-D-glucopyranoside (Vitexin) (1), Quercetin-6-C-β-D-glucopyranoside (2),apigenin-6,8-di-C-β-D-glucopyranoside (Vicenin II) (3), 6-O-methyl Kaempferol 3-O-glucopyranoside (4), luteolin-3-C-gentiobiosyl (5), while two compounds vis; luteolin-6-C-β-Dglucopyranoside (Isoorientin) (6) and luteolin-8-C-β-D-glucopyranoside (Orientin) (7), were isolated for the second time from this species. The ethyl acetate fraction has shown moderate activity against Gram positive and Gram negatvie bacteria, also it showed moderate antioxidant activity with IC50 = 23 ± 0.74 µg/ml when compared to ascorbic acid IC50 = 14.2 ± 0.35 µg/ml. Also ethyl acetate extract has shown cytotoxic activity on MCF-7 cells (human breast cancer cell line), HepG-2 (human hepatocellular carcinoma) and HeLa cells (human cervical cancer cell line), whereas, the methanol extract has shown lower activity. Conclusion: Livistona decipiens have potential medicinal value being rich in polyphenolics and being antioxidant, cytotoxic and antimicrobial drug.

Objectives: This study aimed to compare two Livistona species; Livistona decipiens Becc and Livistona australis Mart for their phenolic and carbohydrate contents and for their protective activity against ulcerative colitis. Methods: A high performance liquid chromatographic (HPLC) technique with inline connected to photo diode array detector (DAD) and electro array (EA) used for detection of the polyphenolic contents. Also HPLC with refractive index detection (HPLC-RI) was used to determine and to quantify carbohydrate contents before and after partial acid hydrolysis, using 0.2 N H 2 SO 4 , for 3 h at 100°C, for each of the defatted methanol extract concentrate. Protective activity against ulcerative colitis was evaluated by acetic acid inducing-ulcers method for both methanol extracts of both species. Results: Both investigated Livistona species are rich in polyphenolic constituents, showing a great similarity. The major flavonoid compound in both species was luteolin-6-C-arabinoside-8-C-glucoside and the major aglycone was acacetin, while the major phenolic acid in both species was ellagic acid. However there was a difference in the carbohydrate content between the two species, the main sugars, before hydrolysis, in L. decipiens were arabinose, mannose and sucrose, while the main sugars in L. australis were glucose, fructose and maltose. However, stachyose was the major polysaccharide obtained after partial acid hydrolysis. L. australis showed protective activity against ulcerative colitis in lower dose 500 mg/kg when compared to L. decipiens that only showed effectiveness at a doubled dose 1000 mg/kg. Conclusion: Both Livistona species have potential medicinal value being rich in polyphenolic and polysaccharide contents and having protective activity against ulcerative colitis.