Purpose: To compare the intestinal absorption of flumequine (FLM) and oxytetracycline (OTC) in encapsulated and non-encapsulated forms in the presence of divalent ions. Methods: MIL-100 (Fe) nanoparticles were synthesized under hydrothermal conditions from a mixture of iron carboxylate and trimesic acid (organic linker), and then used to encapsulate OTC and FLM. Permeation of the various formulations through the mouse jejunum was evaluated in Ussing chamber. Results: There was significant (p ˂ 0.05) increase in the intestinal flux of encapsulated OTCs (OTC-NPs, 0.072 ± 0.016 μg/h/cm 2), compared to that of non-encapsulated OTCs (0.021 ± 0.05 μg/h/cm 2). Moreover, the intestinal flux of encapsulated FLMs (FLM-NPs, 0.045 ± 0.006 μg/h/cm 2) was significantly higher than that of non-encapsulated FLMs (0.004 ± 0.0008 μg/ h/cm 2 , p ˂ 0.05). Conclusion: The intestinal flux of encapsulated antibiotics is significantly enhanced in the presence of MIL-100 (Fe), thereby preventing their chelation by divalent ions in solution, and thus improving their intestinal absorption.