A new approach for the detection and enumeration of Streptococcus macedonicus in cheese was developed. The method which is based on a first screening of cheeses by a PCR assay specific for S. macedonicus followed by plating positive samples on a differential medium (SM medium) was applied to 51 samples derived from PDO and traditional Italian cheeses. Streptococcus macedonicus was found in 16 of the 51 samples examined in the present work. With the exclusion of an Asiago cheese sample in which very high numbers of S. macedonicus (7.13 log CFU g(-1)) were found, the counts of S. macedonicus in SM medium ranged from 2.48 to 4.70 log CFU g(-1). In the same cheeses, total streptococci enumerated onto M17 agar were found at higher concentrations with values up to 7.88 log CFU g(-1). The system developed was particularly useful for the differential count of S. macedonicus in cheese and allowed to evaluate the occurrence of this species within the complex microbial lactic acid bacteria (LAB) population, which is typical of traditional cheeses. Results showed that in the examined cheeses S. macedonicus cannot be considered as a dominant LAB species.

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Social integration is an essential part of the life of every human being, but for people with disabilities, there are many situations in which it is still very low. For instance, in sports and outdoor gaming, there is a barrier between players with and without disabilities. Individuals with disabilities play sports almost exclusively with disabled players, not only during official events such as Paralympic games but also in occasional sports groups, while the mixing of people with and without disabilities in sports activities is a key factor of social inclusion. In order to allow a person with motor-skill impairments to play on the same ground as their non-disabled peers, we developed a novel piece of Assistive Technology that lets a person with motor-skill impairments to control a system acting as a goalkeeper during a non-professional football match, with approximately the same performances as a goalkeeper without motor-skill impairments. This electro-mechanical system is composed of a three-meter-long metal guideline and a human-shaped dummy sliding along it. The system is equipped with a high-torque battery-powered direct-current motor and it is controlled by means of electronic boards and sensors to ensure safety and good usability also for players with severe mobility impairments. The results of a pilot testing demonstrated the robustness and high degree of usability of the system, enabling people with motor-skill impairments to competitively participate in matches with non-disabled peers.