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Starch polymer matrices were developed with the incorporation of 1% clove essential oil (EO) () aiming for use as active packaging for sausages. At the concentration of 1% EO in the polymer matrix, it showed exponential behavior with respect to oil release over 30 days, with faster release in the beginning and a tendency towards a reduction in release velocity over time. The presence of OE in the biofilm led to significant differences versus the control in terms of aroma and flavor parameters. It was found that EO had an antioxidant effect in sausages with a significant difference between treatments with respect to TBA (thiobarbituric acid) values at the end of a 15 day period of refrigerated storage. There were no significant variations in pH and Aw among treatments during the evaluated period. A significant negative correlation (-0.78) between brightness (L*) and the lipid oxidation of the products was observed.

ABSTRACT:The use of natural compounds is a less aggressive alternative for the control of insects in stored grains, in relation to synthetic chemical agents. Plants with insecticidal properties can be used as a source of these compounds to the direct application in pest control. In this work, the essential oil of Cinnamodendron dinisii was chemically characterized and tested regarding its insecticidal and repellent effect on the control of Sitophilus zeamais in stored grains. The essential oil was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). The insecticidal potential was evaluated through the maintenance of the insects during 24 hours in contact with several doses of the oil, in the absence of feed substrate. The Bioassays of repellency were conducted with lethal doses (LD 5 , LD 25 , LD 50 and LD 95 ) obtained from insecticidal bioassay. In order to compare the treatments, the preference index (PI) was used. The essential oil of C. dinisii had insecticidal activity against S. zeamais, causing a linear and crescent mortality with LD of 0.04, 0.17, 0.34 and 0.63 µL/cm 2 , respectively. The repellency ranged from 55.4% to 85.2%, using the LD values previously mentioned. The DL 5 was neutral regarding repellence (PI index -0,09), but from DL 25 on, the PI index was between -0.1 and -1.0, indicating repellence activity.Keywords: essential oil, insecticidal, repellence, Sitophilus zeamais. RESUMO: Óleo essencial de Cinnamodendron dinisii Schwanke para controle deSitophilus zeamais Motschulsky (Coleoptera: Curculionidae). O uso de compostos naturais é uma alternativa menos agressiva para o controle de insetos em grãos armazenados em relação aos agentes químicos sintéticos. Plantas com propriedades inseticidas podem ser usadas como fonte destes compostos para a aplicação direta no controle de pragas. No presente trabalho, o óleo essencial de Cinnamodendron dinisii foi caracterizado quimicamente e testado em relação ao seu efeito repelente inseticida no controle de Sitophilus zeamais em grãos armazenados. O óleo essencial foi obtido por hidrodestilação e analisado por cromatografia gasosa-espectrometria de massa (GC-MS). O potencial inseticida foi avaliado pela manutenção dos insetos durante 24 horas em contato com várias doses de óleo, na ausência de substrato alimentar. Os bioensaios de repelência foram realizados com as doses letais (DL 5 , DL 25 , DL 50 e DL 95 ) obtidas do bioensaio inseticida. Para comparar os tratamentos foi utilizado o índice de preferência (PI). O óleo essencial de C. dinisii apresentou atividade inseticida sobre S. zeamais, causando mortalidade linear e crescente com DL de 0,04, 0,17, 0,34 e 0,63 µL/cm 2 , respectivamente. A repelência variou entre 55,4% até 85,2%, utilizando os valores de DL acima mencionados. A DL 5 mostrou-se neutra em relação à repelência (índice PI -0,09), mas a partir de DL 25 o índice PI foi entre -0,1 e -1,0, indicando atividade de repelência.Palavras-chave: óleo essencial, inseticida, repelência, Sitophilus zeamais.

No abstract

Dye and proteins recovered from broiler blood were used in the formulation of chicken sausages in substitution of soy proteins and synthetic dyes. Three formulations of chicken sausages were prepared: standard, liquid plasma, and freeze-dried plasma. The application of protein and blood dye in the formulation of sausages and sensory analysis followed the Brazilian legislation. Physicochemical, microbiological and sensory attributes of sausages were evaluated during 60. All the formulations met the physicochemical and microbiological requirements. Considering the sensory analysis, sausages prepared using freeze-dried plasma presented significant difference from the standard sausage. There was no significant difference in the acceptance between the standard and liquid plasma sausages. Therefore, the use of such byproducts can add value to meat products without any adverse effect consumers acceptability.