Conventional anastomoses with interrupted sutures are challenging and inevitably associated with trauma to the vessel walls. The goal of this study was to evaluate a novel alternative adhesive-based suture-free anastomosis technique that uses an intraluminal stent. Overall, 120 porcine coronary vessels were analyzed in an ex vivo model and were examined for their mechanical (n = 20 per cohort) and hydrostatic strength (n = 20 per cohort). Anastomoses were made using the novel VIVO adhesive with an additional intraluminal nitinol stent and was compared to interrupted suture anastomosis and to native vessels. Sutureless anastomoses withstood pressures 299 ± 4.47 [mmHg] comparable to native vessels. They were performed significantly faster 553.8 ± 82.44 [sec] (p ≤ 0.001) and withstood significantly higher pressures (p ≤ 0.001) than sutured anastomoses. We demonstrate that the adhesive-based anastomosis can also resist unphysiologically high longitudinal tensile forces with a mean of 1.33 [N]. Within the limitations of an in vitro study adhesive-based suture-free anastomosis technique has the biomechanical potential to offer a seamless alternative to sutured anastomosis because of its stability, and faster handling. In vivo animal studies are needed to validate outcomes and confirm safety.

Tissue Engineering is considered a promising route to address existing deficits of autografts and permanent synthetic prostheses for tendons and ligaments. However, the requirements placed on the scaffold material are manifold and include mechanical, biological and degradation-related aspects. In addition, scalable processes and FDA-approved materials should be applied to ensure the transfer into clinical practice. To accommodate these aspects, this work focuses on the high-scale fabrication of high-strength and highly oriented polycaprolactone (PCL) fibers with adjustable cross-sectional geometry and degradation kinetics applying melt spinning technology. Four different fiber cross-sections were investigated to account for potential functionalization and cell growth guidance. Mechanical properties and crystallinity were studied for a 24-week exposure to phosphate-buffered saline (PBS) at 37 °C. PCL fibers were further processed into scaffolds using multistage circular braiding with three different hierarchical structures. One structure was selected based on its morphology and scaled up in thickness to match the requirements for a human anterior cruciate ligament (ACL) replacement. Applying a broad range of draw ratios (up to DR9.25), high-strength PCL fibers with excellent tensile strength (up to 69 cN/tex) could be readily fabricated. The strength retention after 24 weeks in PBS at 37 °C was 83–93%. The following braiding procedure did not affect the scaffolds’ mechanical properties as long as the number of filaments and the braiding angle remained constant. Up-scaled PCL scaffolds resisted loads of up to 4353.88 ± 37.30 N, whilst matching the stiffness of the human ACL (111–396 N/mm). In conclusion, this work demonstrates the fabrication of highly oriented PCL fibers with excellent mechanical properties. The created fibers represent a promising building block that can be further processed into versatile textile implants for tissue engineering and regenerative medicine.

Braids are generally divided into 2D braids and 3D braids. Two-dimensional braids include flat braids and circular braids. Circular braids represent three-dimensional textiles, as they enclose a volume, but consist of a two-dimensional yarn architecture. Three-dimensional braids are defined by a three-dimensional yarn architecture. Historically, 3D braids were produced on row and column braiding machines with Cartesian or radial machine beds, by bobbin movements around inlay yarns. Three-dimensional rotary braiding machines allow a more flexible braiding process, as the bobbins are moved via individually controlled horn gears and switches. Both braiding machines at the Institut für Textiltechnik (ITA) of RWTH Aachen University, Germany, are based on the principal of 3D rotary machines. The fully digitized 3D braiding machine with an Industry 4.0 standard enables the near-net-shape production of three-dimensionally braided textile preforms for lightweight applications. The preforms can be specifically reinforced in all three spatial directions according to the application. Complex 3D structures can be produced in just one process step due to the high degree of design freedom. The 3D hexagonal braiding technology is used in the field of medical textiles. The special shape of the horn gears and their hexagonal arrangement provides the densest packing of the bobbins on the machine bed. In addition, the lace braiding mechanism allows two bobbins to occupy the position between two horn gears, maximizing the number of bobbins. One of the main applications is the near-net-shape production of tubular structures, such as complex stent structures. Three-dimensional braiding offers many advantages compared to 2D braiding, e.g., production of complex three-dimensional geometries in one process step, connection of braided layers, production of cross-section changes and ramifications, and local reinforcement of technical textiles without additional process steps. In the following review, the latest developments in 3D braiding, the machine development of 3D braiding machines, as well as software and simulation developments are presented. In addition, various applications in the fields of lightweight construction and medical textiles are introduced.