Objective: To investigate the effect of propofol on invasion and metastasis of A549 cells and the expression of intercellular adhesion molecule 1 (ICAM-1) / matrix metalloproteinase-9 (MMP-9) axis.Methods: A549 cells were treated with gradient doses of propofol (25, 50, 100 ng/ml) and different times (12, 24 h). The influences of propofol on ICAM-1 mRNA of A549 cells were observed by qRT-PCR, and the roles of different concentrations of propofol on ICAM-1 and MMP-9 protein and invasion ability were detected by Western Blot and Transwell assay, respectively.Results: The maximum inhibitory influence of propofol was achieved in group 100μg/ml for 24 h. ICAM-1 and MMP-9 protein in the treatment group with 25, 50 and 100μg/ml propofol for 24 h were significantly lower than those in the control group. The number of migrated and invaded cells in the 25, 50 and 100 ng/ml propofol treatment group for 24 h was significantly lower than that in the blank group.Condusion: ICAM-1 protein and MMP-9 protein in human lung cancer A549 cells could be down-regulated after treatment with 50 and 100μg/ml propofol, and the invasion and metastasis ability of A549 cells could be attenuated through ICAM-1/MMP-9 axis.

Background: By analyzing the risk factors of postoperative complications in elderly patients with hip replacement, We aimed to develop a nomogram model based on preoperative and intraoperative variables and verified the sensitivity and specificity for risk stratification of postoperative complications in elderly with total hip replacement patients.Methods: A total of 414 elderly patients who underwent surgical treatment for total hip replacement hospitalized at the Affiliated Hospital of Guangdong Medical University from March 1, 2017 to August 31, 2019 were included into this study. Univariate and multivariate logistic regression were conducted to identify independent risk factors of postoperative complication in the 414 patients. A nomogram was developed by R software and validated to predict the risk of postoperative complications.Results: Multivariate logistic regression analysis revealed that age (OR=1.05, 95%CI: 1.00-1.09) , renal failure(OR=0.90, 95% CI: 0.83～0.97) , Type2 diabetes (OR=1.05, 95% CI: 1.00～1.09) , ALB (OR=0.91, 95% CI: 0.83～0.99) were independent risk factors of postoperative complication in elderly patients with hip replacement (P＜0.05) . For validation of the nomogram, ROC curve revealed that the model predicting postoperative complication in elderly patients with hip replacement was the area under the curve of 0.8254(95% CI: 0.78～0.87) , the slope of the calibration plot was close to 1 and the model passed Hosmer-Lemeshow goodness of fit test (x2 = 10.16, P=0.4264), calibration in R Emax=0.176, Eavg=0.027, which all demonstrated that the model was of good accuracy. Conclusion: The nomogram predicting postoperative complications in patients with total hip replacement constructed based on age, type 2 diabetes, renal failure and ALB is of good discrimination and accuracy, which was of clinical significance.

Background A nomogram(arthro-MAP) predict major complications after Hip and Knee Arthroplasty has been establish by England people. The goal of this study is to externally validate the nomogram whether suitable for Chinese people or not. Methods A total of 414 elderly patients with Hip and Knee Arthroplasty underwent surgical treatment in the Affiliated Hospital of Guangdong Medical University from March 1, 2017 to August 31, 2019 were included into this study. Variables assessed were lowest intraoperative HR, EBL, preoperative BUN, procedure type, race, ASA score, comorbidities, presence of fracture. Receiver-operator characteristics (ROC) curve analysis, calibration (HL) and DCA were used to evaluate predictive properties of the nomogram. Results A total of 414 patients were consecutively enrolled. 47 patients (11.35%) presented a major complications after Hip and Knee Arthroplasty. The arthro-MAP nomogram presented an AUC of 0.816; 95%CI: 0.757–0.875 to predict major complications. At the calibration plot x2 = 17.13, P > 0.05, means the arthro-MAP nomogram appears to be well-calibrated in Chinese population, with predicted outcome rates closely reflecting the observed rates. Conclusions Although further studies are needed to confirm our results, the arthro-MAP nomogram was, in our experience, an excellent tool to predict major complications after Hip and Knee Arthroplasty, can be perform well in the Chinese population.