Aim: This report was aimed to investigate both the chemical composition and the antioxidant activity of two extracts from Mentha rotundifolia aerial parts. Methods: Aqueous and acetonic extracts were obtained by decoction and Soxhlet apparatus, respectively. Total polyphenols contents were determined using Folin-Ciocalteu reagent whereas flavonoids were evaluated by AlCl 3 method. LC-MS/MS method was carried out to reveal the phytochemical composition of extracts. Some tests were used to evaluate the in vitro antioxidant activity of extracts such as DPPH free radical scavenging assay, ABTS + radical cation decolorization assay, OH • radical scavenging assay, ferrous ion chelating, reducing power and β-caroten/linoleic acid bleaching assay. Results: The results showed that the aqueous extract was richer on polyphenols and flavonoids comparing to the acetonic extract. The analysis by LC-MS/MS allowed to identify many phenolic compounds in extracts, predominated by rosmarinic acid. In addition, the extracts were found to possess a significant antioxidant activity. Conclusion: Accordingly, it can be concluded that M. rotundifolia is quite rich in phenolic compounds and has a good antioxidant activity.

Lamium amplexicaule. L is a plant of the Lamiaceae family, widespread in Algeria and North Africa. In the literature, no detailed research elucidating the methods of extraction of phenolic compounds from this species is available. Therefore, the objective of this study was to evaluate the efficient method between cold and hot extraction conditions. Ethanolic extracts were obtained by maceration and Soxhlet apparatus. Phytochemical composition was determined by liquid chromatography mass spectrometry (LC-MS/MS) method. Results demonstrate that the major compound in both extracts was chlorogenic acid. However, 18 compounds were identified in the extract obtained by Soxhlet and 17 in that obtained by maceration. The quantification of these constituents showed higher amounts in the Soxhlet extract than the maceration one. Therefore, it can be concluded that the Soxhlet extraction is more efficient for L. amplexicaule than the maceration one.