RESUMOApesar de o Brasil se destacar como um dos principais produtores mundiais de mamão, ainda existe um reduzido número de linhagens/híbridos disponíveis para comercialização. O presente trabalho objetivou selecionar linhagens entre e dentro de progênies em uma população segregante de mamoeiro para avanço das autofecundações. Originalmente a população foi desenvolvida via retrocruzamento objetivando a incorporação (característica Golden) para tolerância à mancha fisiológica nos genitores do híbrido UENF/CALIMAN01, motivo pelo qual foi utilizado um índice de seleção denominado IG2 amparado nos valores genotípicos individuais obtidos via modelos mistos, pelo procedimento REML/BLUP. O resultado da análise permitiu selecionar oito progênies que segregaram para a característica "tipo Golden": 11, 13, 14, 15, 16 19, 18 e 12; dentro das progênies as plantas apresentaram valores superiores de ganho e média predita em relação às testemunhas (JS12, SS72/12 e Golden) para as seguintes características: altura de inserção do primeiro fruto, firmeza da polpa e do fruto e produção. Foram selecionadas 20 plantas com a característica "tipo Golden" visando ao avanço de geração de autofecundação e retrocruzamento. Palavras-chave:Carica papaya L., índice de seleção, REML/BLUP Mixed model for combining selection in segregate progeny of papaya ABSTRACT Although Brazil stands out as a leading global producer of papaya still exists a small number of lines/hybrids available for its cultivation. To obtain new cultivars it is necessary to direct efforts towards the identification and selection of superior genotypes and broadening the genetic basis of the species. This study aimed to select lines between and within families in a segregating population of papaya for advancement of self pollination and backcross. Originally the backcross population aimed the introgression of the physiological disturb (Golden type) into the UENF/CALIMAN01 genitors. For this a selection index named IG2 was used supported in individual genotypic values obtained through mixed models by REML/BLUP. Analysis of results permitted selection of eight progenies and segregated for the trait "Golden Type": 11, 13, 14, 15, 16, 19, 18 and 12. The selected genotypes within each progeny showed higher values of gain and predicted average than the controls (JS12, SS72/12 and Golden) for the following characteristics: height of insertion of the first fruit, firmness of fruit and production. Twenty plants e selected with the characteristic "Golden Type" for the advancement of generation of selfing and backcross.
Melaleuca alternifolia is cultivated for the production of an essential oil useful in the cosmetic and pharmaceutical industries. Despite the economic importance of this species, there is little knowledge about its in vitro propagation. The aim of this study was to establish an efficient protocol for micropropagation of M. alternifolia. With the goal of in vitro multiplication by axillary shoot proliferation, both solid and liquid MS and WPM media were tested with supplementation with BA at 0, 0.55, 1.11, 2.22, 3.33, and 4.44 μM. The best result for shoot multiplication was obtained when either 0.55 μM BA was added into solid MS medium or 1.11 μM BA was added into liquid MS medium, with 5.6 and 11.8 shoots per explant generated, respectively. On solid or liquid WPM medium supplemented with 0.55 μM BA, the proliferation rates were 5.5 and 4.7, respectively. Three auxins (NAA, IAA, and IBA) were tested at 0.53 and 2.64 μM during the rooting stage. Several sucrose concentrations (15, 30, and 45 g L −1 ) were compared to a sucrose-free medium. Rooting performances on four culture media were then compared: MS, half-strength MS (MS/2), MS+activated charcoal (AC), and MS/2+AC. The results showed that auxin addition to culture medium is not necessary for in vitro rooting. Rooted microcuttings from different culture media were acclimatized in a greenhouse, and the survival percentage was evaluated. All shoots cultured in an auxinfree MS medium supplemented with sucrose (30 gL −1 ) produced roots, and all plants survived during acclimatization. Activated charcoal added in rooting medium reduced rooting rates.
RESUMO:Melaleuca alternifolia tem como produto principal o óleo essencial extraído das folhas devido às propriedades antifúngicas e antibacterianas. Pouco se tem relatado sobre a propagação desta espécie, sendo a miniestaquia uma alternativa para a propagação vegetativa de clones superiores visando à implantação de campo de produção. ), em dois tipos de miniestacas (apical e mediana). As miniestacas foram confeccionadas com 5 cm de comprimento, mantidas em casa de vegetação com nebulização intermitente, e, após 45 dias do plantio, foram avaliadas as porcentagens de miniestacas enraizadas, com calos e não responsivas, o número de raízes formadas por miniestaca e o comprimento das três maiores raízes (cm). O substrato Golden-Mix ® e as miniestacas coletadas da porção apical do ramo submetidas ao tratamento com 500 mg L -1 de AIB apresentaram maior porcentagem de enraizamento e melhor qualidade do sistema radicial. Palavras-chave:Myrtaceae, auxina, óleo essencial, plantas medicinais, propagação vegetativa ABSTRACT: Substrates, indolebutyric acid levels and types of minicuttings on the rooting of tea tree (Melaleuca alternifolia Cheel). Melaleuca alternifolia has as major product the essential oil extracted from its leaves due to its antifungal and antibacterial properties. There are scarce reports about the propagation of this species, and minicutting is an alternative for vegetative propagation of superior clones in order to establish a production field. This study aimed to evaluate the effect of different substrates, IBA levels and types of minicuttings on the rooting of Melaleuca alternifolia. In the first experiment, the following substrates were tested: medium sand, Plantmax HT ® , Golden-Mix ® and vermiculite. In the second experiment, different IBA levels (0, 500, 1000 and 2000 mg L -1 ) were tested for two minicutting types (apical and medium). Minicuttings were prepared with 5 cm length and were kept in a greenhouse with intermittent mist; then, at 45 days after planting, we evaluated: the percentages of rooted minicuttings, with callus and non-responsive, the number of roots per minicutting and the length of the three longest roots. The substrate Golden-Mix ® and the minicuttings collected from the apical part of the branch and treated with 500 mg L -1 IBA presented the largest rooting percentage and the best root system quality.
RESUMOMelaleuca alternifolia Cheel (Myrtaceae), também conhecida por "tea tree", é uma árvore nativa da Austrália com grande importância econômica devido a extração do óleo essencial de suas folhas, que é utilizado na indústria farmacêutica por possuir comprovada ação antimicrobiana e antifúngica. O principal problema encontrado para a expansão do cultivo de Melaleuca alternifolia é a produção de mudas, já que estudos sobre a propagação desta espécie são escassos. Este trabalho teve como objetivo avaliar a influência de diferentes comprimentos de estaca no enraizamento da espécie. Para tanto, estacas caulinares apicais foram confeccionadas com 10, 15 e 20 cm de comprimento, cortadas em bisel na base, mantendo-se um terço das folhas na porção apical, sendo as bases das mesmas tratadas com 1500 mg L -1 de AIB. Para o plantio foram utilizados tubetes de polipropileno com capacidade de 53 cm 3 , contendo substrato Plantmax HT ® . As estacas foram mantidas em casa-de-vegetação com nebulização intermitente e, após 120 dias do plantio, foram avaliadas as variáveis: porcentagem de enraizamento, porcentagem de sobrevivência, número médio de raízes por estaca e comprimento médio das três maiores raízes por estaca (cm). Pôde-se concluir que estacas de M. alternifolia com 10 cm apresentam maior porcentagem de enraizamento em relação a estacas com 15 e 20 cm.Palavras-chave: "Tea tree"; estaquia; planta medicinal; produção de mudas; Melaleuca alternifolia. ABSTRACTMelaleuca alternifolia Cheel (Myrtaceae), also known as "tea tree", is a native tree of Australia with great economic importance due to extraction of essential oils from its leaves. This oil is used in the pharmaceutical industry for its antimicrobial and antifungical action. The main problem for the expansion of the culture of Melaleuca alternifolia is the production of plants and studies on the vegetative propagation of this species are scarce. The objective of this study was to evaluate the influence of three lengths of cutting on the adventitious rooting. Apical cuttings were prepared with 10, 15 and 20 cm of length (measured from the apex to the base), cut in bevel at the base. One third of the leaves was kept in the apical portion and the cuttings were treated with 1500 mg L -1 IBA. For the plantation under greenhouse, tubes of polypropylene with capacity of 53 cm 3 and containing Plantmax HT ® substrate were used. The cuttings were kept in greenhouse under mist. After 120 days, the percentage of adventitious rooting and survival, the average number of roots per cutting and the average length of the three main roots per cutting were evaluated. In conclusion, 10 cm cuttings of M. alternifolia show higher rooting percentage than 15 or 20 cm cuttings.
RESUMOO abacaxi ornamental (Ananas bracteatus Schult. f.) é bastante utilizado em composições paisagísticas para delimitar áreas ou canteiros. A micropropagação permite obter altas taxas de multiplicação, podendo ser uma alternativa viável e vantajosa de propagação vegetativa, visto que a demanda por esta espécie vem aumentando, tanto no mercado nacional quanto no internacional. Neste trabalho objetivou-se avaliar a influência de diferentes irradiâncias e concentrações de sacarose durante o enraizamento in vitro, sobre a sobrevivência das mudas na fase de aclimatização. Para tanto, foram testadas diferentes concentrações de sacarose (0, 10, 20 e 30 g L -1 ) no meio de enraizamento, bem como duas irradiâncias (22,9 e 66,9 mmol m -2 s -1 ) em sala de crescimento. O melhor resultado em termos de enraizamento foi obtido no meio de cultura contendo 30 g L -1 de sacarose sob irradiância de 66,9 mmol m -2 s -1 . Após a fase de enraizamento in vitro, as plantas foram transferidas para casa de vegetação e mantidas sob nebulização intermitente por 60 dias. Os resultados de sobrevivência aos 30 e 60 dias não diferiram estatisticamente para os dois níveis de irradiância testados. Entretanto, para concentrações de sacarose, a testemunha apresentou sobrevivência inferior (50%) diferindo estatisticamente dos demais tratamentos que foram semelhantes entre si (95 a 100%). Termos para indexação:Ananas bracteatus, micropropagação, planta ornamental. ABSTRACTOrnamental pineapple (Ananas bracteatus Schult. f.) is often used in landscape compositions in order to limit areas or borders. Micropropagation allows high multiplication rates and may be an interesting technique of vegetative propagation in order to meet the need of plants of this species that is rising on national and international markets. The objective of this study was to determine the effect of several light irradiances and sucrose concentrations during in vitro rooting on survival during the acclimatization stage. Several concentrations of sucrose (0, 10, 20 or 30 g L -1 ) in the rooting medium and two light irradiances (22.9 and 66.9 mmol m -2 s -1 ) in the culture room were tested. The best results of rooting were obtained on culture medium containing 30 g L -1 sucrose under an irradiance of 66.9 mmol m -2 s -1 . The plants were then transferred to the greenhouse and cultured under intermittent mist for 60 days. There was no significant difference among the results of survival under the two irradiances tested. However, for sucrose concentrations, the survival rate of the control (50%) was statistically inferior to that of the other treatments that were similar among them (95 and 100%).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.