En 330 muestras de ADN de ocho razas bovinas criollas (Blanco Orejinegro, Caqueteño, Casanareño, Costeño con Cuernos, Chino Santandereano, Hartón del Valle, Romosinuano y Sanmartinero), dos razas sintéticas colombianas (Lucerna y Velásquez) y dos foráneas (Brahmán y Holstein) se evaluó la presencia del VLB (detección de provirus -PCR anidada), los polimorfismos del gen BoLA-DRB3.2* (PCR semianidada -RFLP) y la asociación entre ambos (OR). Se estimaron asociaciones entre la ausencia (resistentes) del VLB y los alelos *21, *24 y *37 y la presencia (susceptibles) del VLB y los alelos *6 y *42 en los ganados criollos. La frecuencia acumulada de los alelos resistentes fue de 23.7% contra 6% de los susceptibles. El 10% de los individuos fue genotipificado como Resistente/ Resistente, el 2.5% como Susceptible/Susceptible y el 57% fue de genotipo homocigoto neutral (N/N) en el ganado criollo colombiano. En los ganados controles el 16% fueron Resistente/Resistente y el 8.3% Susceptible/Susceptible. Los resultados indican que el ganado criollo colombiano posee genes de resistencia al VLB.Bovinos criollos, Complejo mayor de Histocompatibilidad, Marcadores moleculares, Resistencia a enfermedades The presence of the bovine leukemia virus (BLV) (pro-virus detection -Nested PCR), BoLA-DRB3.2* gen polymorphisms detection (Semi-nested PCR-RFLP) and the association between them (OR) were carried out in 330 DNA samples from eight creole breeds (Blanco Orejinegro, Caqueteño, Casanareño, Costeño con Cuernos, Chino Santandereano, Hartón del Valle, Romosinuano and Sanmartinero), two Colombian synthetic breeds (Lucerna y Velásquez) and two introduced ones (Brahmán y Holstein). A positive association was found between absence of BLV (resistant individuals) with alleles *21, *24 and *37; and the presence of BLV (susceptible individuals) with alleles *6 and *42. The cumulated allele frequency was 23.7% and 6% for the resistant and susceptible alleles respectively. For the Colombian creole cattle, 10% of genotyped individuals were classified as Resistant/ Resistant (RR), 2.5% as Susceptible/Susceptible (SS) and 57% as neutral homozygous (N/N). For the introduced breeds, 16% were RR and 8.3% SS. The results indicate that the Colombian creole cattle has VLB resistance genes.Creole bovines, M a j o r h i s t o c o m p a t i b i l i t y complex, Molecular markers, Disease resistance.
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