This study was done to partially purification of  topoisomerase IB from serum of diabetic patients using Gel filtration technique, by using Sephadex G 100 gel. A single peak in fraction four has been obtained, and the degree of purification (17.1) fold, enzyme yield (108.2%) and specific activity (0.189ng/mg). Kinetics studies for the partial purified enzyme were carried out which showed optimal concentration of  substrate which was (0.1ng/ml), Michael's - Menten constant (Km=0.033ng) and maximum velocity (Vmax=0.90 ng/ml), while optimum Temperature was (37C°) and optimum pH was (7.5). The molecular weight of the partial purified enzyme has been determined by gel electrophoresis method, in presence of polyacrylamide  gel and sodium dodecyl sulphate (SDS-PAGE) which showed that the approximated molecular weight was (66KD).   http://dx.doi.org/10.25130/tjps.23.2018.168

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This study has been acomblished between December 2016 and September  2017 which included 70 patients with chronic renal failure who attended at Al-yarmuke teaching hospital in Baghdad with25 healthy individual as control. The research aims to study the effect of hypertension, diabetes and smoking on the levels of some biochemical parameters of chronic renal failure patients comparing with healthy control .The results showed a significant increase in the  concentrations of urea, creatine, uric acid, cholesterol, Triglycerides(TG), low density lipoproteins (LDL) and Very low density lipoproteins (VLDL), compared to healthy control. The results also showed that hypertension has an effect on the concentration of urea, uric acid, creatinine, cholesterol, Triglycerides (TG), low density lipoproteins (LDL) and Very low density lipoproteins (VLDL), the results indicated that the diabetes has effects on the concentration of urea, creatinine, cholesterol and Triglycerides (TG), High density lipoproteins (HDL), low density lipoproteins( LDL) and Very Low density lipoproteins (VLDL) with no effects on the concentration of uric acid. There was an effect of smoking on The concentrations of cholesterol and the Triglycerides(TG), low Density Lipoproteins( LDL), Very Low density Lipoproteins (VLDL), urea, uric acid, and creatinine .The gender has no effect on the concentration of all measured parameters except on TG concentration   http://dx.doi.org/10.25130/tjps.24.2019.049

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