Bahr Arias M.V., Padilha F.N. & Perugini M.R.E. 2017. Deep tissue culture and hemoculture in dogs with wounds and sepsis. Pesquisa Veterinária Brasileira 37(12):1483-1490. Contaminated and infected wounds occur very frequently in veterinary medicine and can cause systemic inflammatory response syndrome, sepsis, and death. This study aimed to test the feasibility of collecting wound material by deep-tissue or punch biopsy for microbial culture, determine the frequency of bacteria in the wound(s) and blood cultures and the susceptibility of these microbes to antimicrobials, and evaluate clinical parameters that could be related to prognosis. Thirty dogs with wounds and signs of SIRS/sepsis were included in this study. Bacteria were isolated from all wounds and 41 bacterial isolates could be identified based on culture of the materials collected by punch biopsy; 53.66% of the isolates were gram-negative, mainly involving Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterococcus spp., and 46.34% were gram-positive bacteria such as Streptococcus spp., Enterococcus spp., and Staphylococcus spp. The survival rate was 66.67%. Based on blood culture analysis, we identified bacteremia in seven patients, predominantly of gram-negative bacteria, which negatively affected patient survival, as six dogs died. Hypoglycemia (≤60mg/dL) and severe hyperglycemia (≥180mg/dL) also negatively affected survival as 23.33% of the hypo/hyperglycemic dogs died. Factors such as blood lactate level at admission and hematocrit levels, and mean arterial pressure were not significantly correlated with death or survival of the dogs.
O objetivo deste trabalho foi analisar a atividade antiproliferativa do sulfato de vincristina em cultivos de tumores mamários caninos in vitro por meio da determinação de concentração inibitória e estabelecimento do estadiamento clínico. Amostras frescas obtidas de 20 pacientes caninas com neoplasia mamária foram fatiadas e cultivadas individualmente na presença e ausência de sulfato de vincristina durante três dias para determinar a viabilidade e a citotoxicidade. Quinze amostras apresentaram sensibilidade ao sulfato de vincristina proporcional à dose. Amostras com o estágio clínico avançado apresentaram crescimento celular correlacionado ao potencial proliferativo dos tumores malignos de se desenvolverem melhor que os benignos em cultivo celular. Concluiu-se que o método utilizado pode ser adaptado como modelo de aplicabilidadeno estudo de resposta tumoral a drogas antitumorais em cultivos primários.
A síndrome de horner (SH) ocorre devido à alteração na inervação simpática do olho, caracterizada principalmente por uma tétrade de sinais: ptose, miose, enoftalmia, protrusão de terceira pálpebra. Vários fatores podem contribuir para o estabelecimento dessa síndrome como secundária a traumas, infarto, neoplasia ou doenças inflamatórias. As neoplasias em regiões cervicais podem comprimir a inervação simpática do olho, causar metástase em determinadas regiões do corpo, e a alta vascularização, localização e íntima relação do tumor com estruturas nobres (vasos da cavidade torácica e pescoço) tornam o prognóstico desfavorável. Dessa forma, objetiva-se relatar a ocorrência de SH por compressão cervical do tronco vago simpático por quemodectoma carotídeo maligno.
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