Gravity Recovery and Climate Experiment (GRACE) mission is dedicated to measuring temporal variations of the Earth's gravity field. In this study, the Stokes coefficients made available by Groupe de Recherche en Géodésie Spatiale (GRGS) at a 10-day interval were converted into equivalent water height (EWH) for a ~4-year period in the Amazon basin (from July-2002 to May-2006). The seasonal amplitudes of EWH signal are the largest on the surface of Earth and reach ~ 1250mm at that basin's center. Error budget represents ~130 mm of EWH, including formal errors on Stokes coefficient, leakage errors (12 ~ 21 mm) and spectrum truncation (10 ~ 15 mm). Comparison between in situ river level time series measured at 233 groundbased hydrometric stations (HS) in the Amazon basin and vertically-integrated EWH derived from GRACE is carried out in this paper. Although EWH and HS measure different water bodies, in most of the cases a high correlation (up to ~80%) is detected between the HS series and EWH series at the same site. This correlation allows adjusting linear relationships between in situ and GRACE-based series for the major tributaries of the Amazon river. The regression coefficients decrease from up to down stream along the rivers reaching the theoretical value 1 at the Amazon's mouth in the Atlantic Ocean. The variation of the regression coefficients versus the distance from estuary is analysed for the largest rivers in the basin. In a second step, a classification of the proportionality between in situ and GRACE time-series is proposed. KeywoRdS: Spatial geodesy, hydrology, GRACE satellite, Amazon Basin. Variações temporais do equivalente à altura d'água obtidas da Missão Grace e da altura d'água in-situ nos rios da bacia AmazônicaReSUMo A missão espacial Gravity Recovery and Climate Experiment (GRACE) é dedicada às medidas das variações temporais no campo gravitacional da Terra. Neste estudo, os coeficientes de Stokes disponibilizados pelo Groupe de Recherche en Géodésie Spatiale (GRGS) com intervalos de 10 dias foram convertidos no equivalente à altura d'água (EWH) para um período de 4 anos na bacia Amazônica (de julho de 2002 a maio de 2006). As amplitudes sazonais do EWH no centro da bacia são as maiores encontradas no mundo (~1250 mm). Os erros na estimativa do EWH são de ~130 mm, incluindo os erros nos coeficientes de Stokes, erros de vazamento de informações das bacias vizinhas (12 ~ 21 mm) e truncamento do espectro (10 ~ 15 mm). Neste trabalho, são feitas comparações entre as informações de altura d'água in-situ medidas em 233 estações hidrológicas (HS) na bacia Amazônica com EWH obtido pelo GRACE. Embora o EWH e as HS meçam diferentes corpos d'água, na maioria dos casos, uma alta correlação (até 80%) é encontrada entre ambas as séries ao mesmo local. Esta alta correlação permite ajustar uma relação linear entre as séries para os maiores tributários do rio Amazonas. O coeficiente de regressão decresce da montante para a vazante, tendendo para o valor teórico 1 na foz do rio Amazonas. A variação des...

Road networks are monitored to evaluate their decay level and the performances regarding ride comfort, vehicle rolling noise, fuel consumption, etc. In this study, a novel inertial sensor-based system is proposed using a low-cost inertial measurement unit (IMU) and a global positioning system (GPS) module, which are connected to a Raspberry Pi Zero W board and embedded inside a vehicle to indirectly monitor the road condition. To assess the level of pavement decay, the comfort index awz defined by the ISO 2631 standard was used. Considering 21 km of roads with different levels of pavement decay, validation measurements were performed using the novel sensor, a high performance inertial based navigation sensor, and a road surface profiler. Therefore, comparisons between awz determined with accelerations measured on the two different inertial sensors are made; in addition, also correlations between awz, and typical pavement indicators such as international roughness index, and ride number were also performed. The results showed very good correlations between the awz values calculated with the two inertial devices (R2 = 0.98). In addition, the correlations between awz values and the typical pavement indices showed promising results (R2 = 0.83–0.90). The proposed sensor may be assumed as a reliable and easy-to-install method to assess the pavement conditions in urban road networks, since the use of traditional systems is difficult and/or expensive.

Accurate and rapid determination of trypanosomatids is essential in epidemiological surveillance and therapeutic studies. Matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) has been shown to be a useful and powerful technique to identify bacteria, fungi, metazoa and human intact cells with applications in clinical settings. Here, we developed and optimized a MALDI-TOF MS method to profile trypanosomatids. trypanosomatid cells were deposited on a MALDI target plate followed by addition of matrix solution. The plate was then subjected to MALDI-TOF MS measurement to create reference mass spectra library and unknown samples were identified by pattern matching using the BioTyper software tool. Several m/z peaks reproducibly and uniquely identified trypanosomatids species showing the potentials of direct identification of trypanosomatids by MALDI-TOF MS. Moreover, this method discriminated different life stages of Trypanosoma cruzi, epimastigote and bloodstream trypomastigote and Trypanosoma brucei, procyclic and bloodstream. T. cruzi Discrete Typing Units (DTUs) were also discriminated in three clades. However, it was not possible to achieve enough resolution and software-assisted identification at the strain level. Overall, this study shows the importance of MALDI-TOF MS for the direct identification of trypanosomatids and opens new avenues for mass spectrometry-based detection of parasites in biofluids. Copyright © 2016 John Wiley & Sons, Ltd.

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