Biocides are currently considered the first line of defense against foodborne pathogens in hospitals or food processing facilities due to the versatility and efficiency of their chemical active ingredients. Understanding the biological mechanisms responsible for their increased efficiency, especially when used against foodborne pathogens on contaminated surfaces and materials, represents an essential first step in the implementation of efficient strategies for disinfection as choosing an unsuitable product can lead to antibiocide resistance or antibiotic–biocide cross-resistance. This review describes these biological mechanisms for the most common foodborne pathogens and focuses mainly on the antipathogen effect, highlighting the latest developments based on in vitro and in vivo studies. We focus on biocides with inhibitory effects against foodborne bacteria (e.g., Escherichia spp., Klebsiella spp., Staphylococcus spp., Listeria spp., Campylobacter spp.), aiming to understand their biological mechanisms of action by looking at the most recent scientific evidence in the field.

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Farm biosecurity management includes a set of practical measures used to prevent and limit the spread of infections to humans and animals. Infections, predominantly caused by zoonotic agents, often occur due to a lack of safety standards monitoring on farms, but also because of the use of inappropriate antimicrobial products leading to bacterial resistance, tolerance to biocides and the emergence antimicrobial-resistant germs. To date, research was mainly focused on studying the antimicrobial resistance in bacterial biofilms and the mechanisms involved in their occurrence. At molecular level, the limited diffusion of biocides in the biofilm matrix, enzyme-mediated resistance, genetic adaptation, efflux pumps, and levels of metabolic activity inside the biofilm are some of the investigated biological mechanisms which can promote antimicrobial resistance in biofilms were also investigated. Interventions, based on the identification of novel antimicrobial compounds, that would exclude the occurrence of bacterial tolerance, including essential oils (oregano, cloves), organic acids (tannic & oleic acid) and natural plant compounds (e.g. alkaloids, flavonoids, tannins and coumarins) were also extensively studied and reviewed given their effectiveness against pathogen-produced biofilms. The aim of this review was emphasize the importance of biosecurity and farm management practices and to assess their impact on bacterial biofilm formation. Furthermore, we present the recent intervention strategies aimed at reducing and combating the formation of bacterial biofilms in livestock farms.

Rehabilitation of polluted soils with petroleum products requires a re-vegetation strategy to obtain a green mass cover that can quickly and efficiently cover the polluted soil. For the gradual recovery of the destroyed soil, it was necessary: 1. adequate soil treatments with fertilizer i.e. sewage sludge and fly ash as amendment, 2. plant species selection, 3. agronomical works in accordance with geographical position and climatic conditions. Fertilizers and fly ash create conditions for plant installation, by nutrients insurance. Our experiment was conducted in pots with 91.73�11.12 [gKg-1 D.M (dry matter)] total petroleum hydrocarbons (TPH) polluted soil, fertilised with sewage sludge and fly ash 60 [tha-1] derived from the burning of fossil fuels in thermal power plants. The selected plant species for bio-remediation is Lolium perenne. The selected plant species Lolium perenne is installed on 50-90% of the land surface giving 8 successive crops of grass in the warm season. The TPH reductions of polluted and treated soil were 38.4-56.3 [%]. The biomass did not bioaccumulate chromium at the detection limit. The amounts of cadmium, lead and zink bioaccumulated in the aerial parts were below acceptable limits. The obtained biomass can be used as animal feed or for bedding in shelters. The soil remediation efficiencies of 91.73 � 11.12 [gKg-1 D.M] were directly proportional to the amount of fly ash used.

Until now, in Romania, a country in southeastern Europe located on the Balkans Peninsula, information regarding the status on A. mellifera honey bee infection by Nosema spp. has not been published. The aims of the present study were to survey the occurrence and identification of Nosema spp. in A. mellifera honeybee colonies from 37 apiaries located in Arad, Caras-Severin and Timis Counties, Romania. Also, based on published literature an update on the distribution of N. ceranae infection among A. mellifera colonies worldwide was performed. Overall, a frequency of 55.1% (389/690) of Nosema infection was observed in the analyzed regions (ranging from 52% to 66%), by light microscopy. By PCR and DNA sequencing, N. ceranae was the only microsporidia identified. Nosema ceranae is clearly a novel, emergent pathogen of A.mellifera with potentially very serious effects on the individual and honeybee�s colonies in Romania. Data obtained provide new and important information on N. ceranae geographic prevalence and distribution, and on its impact at colony level and/or its role in colony losses. The present study intends to contribute to highlight the importance of implementing prevention, treatment and control measures of honeybee nosemosis, in Romania.