The aim of the investigation was to assess histologically the effect of laser photobiomodulation (LPBM) on a repair of defects surgically created in the femurs of rats. Forty-five Wistar rats were divided into four groups: group I (control); group II (LPBM); group III (hydroxyapatite guided bone regeneration; HA GBR); group IV (HA GBR LPBM). The animals in the irradiated groups were subjected to the first irradiation immediately after surgery, and it was repeated every day for 2 weeks. The animals were killed 15 days, 21 days and 30 days after surgery. When the groups irradiated with implant and membrane were compared, it was observed that the repair of the defects submitted to LPBM was also processed faster, starting from the 15th day. At the 30th day, the level of repair of the defects was similar in the irradiated groups and those not irradiated. New bone formation was seen inside the cavity, probably by the osteoconduction of the implant, and, in the irradiated groups, this new bone formation was incremental. The present preliminary data seem to suggest that LPMB therapy might have a positive effect upon early wound healing of bone defects treated with a combination of HA and GBR.

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The aim of this study was to assess the effect of LLLT (λ830nm, Thera lase, DMC Equipamentos, São Carlos, SP, Brazil, 40mW, CW, spot size 0.60mm, 16J/cm 2 per session) on the repair of bone defects on the femur of Wistar albinus rats which were grafted with anorganic bovine bone associated or not to bovine bone membrane. Five randomized groups were studied: I (Control); II (anorganic bovine bone); III (anorganic bovine bone + LLLT); IV (anorganic bovine bone + bovine bone membrane) and V (anorganic bovine bone + bovine bone membrane + LLLT). The animals were irradiated at every 48h during 15 days, the first irradiation was performed immediately after the procedure. The animals were irradiated transcutaneuosly in four points around the defect. At each point a dose of 4J/cm 2 was given totaling 16J/cm 2 per session. The animals were sacrificed 15, 21 and 30 days after surgery. The specimens were routinely processed to wax and stained with H&E and Picrosírius stains and analyzed under light microscopy. The results showed evidence of a more advanced repair on the irradiated groups when compared to non-irradiated ones. The repair of irradiated groups was characterized by both increased bone formation and amount of collagen fibers around the graft within the cavity early, considering the osteoconductive capacity of the anorganic bovine bone and the increment of the cortical repair in specimens with membrane. It is concluded that LLLT had a positive effect on the repair of bone defect submitted the implantation of graft associated or not to the use of biological membrane.

SThe aim of the present investigation was to assess histologically the effect of LLLT (λ830nm, Thera Lase, DMC, São Carlos, SP, Brazil, 40mW, CW, spot size 0.60mm, 16J/cm 2 per session) on the repair of surgical defects created in the femur of the Wistar Albinus rat. The defects were filled to lyophilized organic bovine bone associated or not to GTR (decalcified cortical osseous membrane). Surgical bone defects were created in 42 animals divided into five groups: Group I (control -6 animals); Group II (organic bovine bone -9 animals); Group III (organic bovine bone + LLLT -9 animals); Group IV (organic bovine bone + decalcified cortical osseous membrane -9 animals); Group V (organic bovine bone + decalcified cortical osseous membrane + Laser -9 animals). The animals on the irradiated groups received 16J/cm 2 per session divided into four points around the defect (4J/cm 2 ) being the first irradiation immediately after surgery and repeated seven times at every 48h. The animals were humanely killed after 15, 21 and 30 days. The results of the present investigation showed histological evidence of improved amount of collagen fibers at early stages of the bone healing (15 days) and increased amount of well organized bone trabeculae at the end of the experimental period (30 days) on irradiated animals compared to non irradiated ones. It is concluded that a positive biomodulative effect on the healing process of one defect associated or not to the use of organic lyophilized bone and biological bovine membrane on the femur of the rat. Downloaded From: http://proceedings.spiedigitallibrary.org/ on 06/25/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx Proc. of SPIE Vol. 4950 159 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 06/25/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx