The changes in weaving technology, competition with synthetic fibers, and the globalization of cotton and textile production have increased the demand for better quality fibers. One of the main traits to be improved is fiber length. Thus, under the hypothesis of distinction and characterization of cotton genotypes, the aim of this study was to evaluate, in three locations, the average behavior of elite breeding lines selected for long fiber, as the agronomic and fiber technological trait. It were evaluated lines derived from a cross between the Guazuncho2 of Gossypium hirsutum L. with Acala SJ4 materials of fiber long in three trials located in Apodi-RN in 2013 and 2014 and Santa Helena-GO in 2013. The experimental design was a randomized complete block with two replications. The following traits were evaluated: plant height, average boll weight, percentage of fiber, cotton productivity in seed, fiber length, fiber uniformity, short fiber index, fiber strength, elongation, maturity of the fibers, micronaire, reflectance and the degree of yellowing. The genotypes; Centro Nacional de Pesquisa de Algodão (CNPA) 2012 to 55 and CNPA 2012 to 58 gather high yield and desired fiber characteristics.