Effects of reabsorption of chlorophyll fluorescence were investigated in measurements on chloroplasts and higher plant leaves. The excitation was perform ed at 650 nm in a typical excitation/detection arrangement; with increasing chlorophyll concentrations, the measured spectral distribution of the chlorophyll fluorescence was significantly distorted, but no effects on the picosecond decay were observed, when this was detected at 685 nm.

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Chlorella vulgaris, Chlorophyll Fluorescence Quenching, Green Algae, Molecular Oxygen Molecular oxygen can act as a collisional quencher of the singlet excited state of chlorophyll a. This effect is well described for chlorophyll a in various solvents but not for chlorophyll a in the antenna complexes of photosynthetic organisms. We studied the chlorophyll fluorescence decay of Chlorella vulgaris cells under different oxygen concentrations but did not find any evidence for quenching by oxygen.