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Most injection-moulded plastics are injection moulded into moulds made from conventional materials such as steel or aluminium. The production costs of the mould are considerable. 3D printing from plastic can be used for injection moulds to save these costs. This article deals with injection moulding into a 3D-printed plastic mould. The injection insert was produced on a HP Multi Jet Fusion 4200 3D printer. The other part of the mould was made of aluminium. A custom injection mould was designed for the research. One insert was made from plastic, and one from aluminium. Both moulds were injected under the same injection conditions. A comparison of injection moulding into the plastic and aluminium inserts is made in this article. The difference when injection moulding into the plastic insert is explained using the different technological conditions. The part injected into the plastic insert was also different from the part injected into the aluminium insert. The difference is explained in this article. This article also looks at the interface between the injection-moulded part and the plastic insert using an electron microscope. The images taken clarify the differences between injection moulding into a plastic insert and an aluminium insert and the differences of the injection-moulded part from the plastic insert.