Background: Oxidative Stress evaluation is fast becoming a diagnostic tool in many disease conditions affecting man. This study evaluated oxidative stress on alloxaninduced diabetic rats by measuring serum Gamma-Glutamyl Transferase (GGT). Total Antioxidant Content (TAC), Lipid Profile, Fasting Plasma Glucose, Transaminases (ALT, AST). Methods: 40 albino Wistar rats of both sexes weighing 190±20g were used for this study. The animals were randomly assigned into two groups A and B consisting of twenty rats each. Group A was the control (non-diabetic) group, while Group B was the experimentally-induced diabetic group. The study lasted for five weeks and blood samples were collected at the third and fifth weeks for some biochemical studies. The following biochemical parameters: Fasting Plasma Glucose (FPG) Serum Urea Creatinine, Alanine and Aspartate Transaminases (ALT, AST) Lipid profile, Gamma-Glutamyl Transferase (GGT) and Total Anti-oxidant Content (TAC) were measured according to standard methods. Histological examinations of major organs-heart, kidneys, and liver-were made according to standard methods. Results: There were significant differences between the groups in the biochemical parameters (p>0.05) and the histological examinations at weeks 3 and 5. The mean serum GGT concentration of the diabetic group at 3 and 5 weeks (4.0±0.2iu/l and 4.4±0.2iu/l respectively) were significantly increased (p<0.001) when compared to the control group 0.8±0.2iu/l and 0.8±0.1iu/l at 3 and 5 weeks respectively while the mean serum TAC of the diabetic group at 3 and 5 weeks (0.8±0.1mmol/l and 0.6±0.1mmol/l) respectively were significantly decreased (p<0.001) when compared to the control 1.6±0.1mmol/l and 1.7±0.1mmol/l at 3 and 5 weeks respectively. The mean Urea, Creatinine, ALT, AST, TC, LDL-C, VLDL-C, TG, FPG and HbA1c values were significantly increased (p<0.001) in the diabetic group when compared to the control group while the mean serum HDL-C value of the diabetic group decreased significantly (p<0.001) when compared with the control at 3 and 5 weeks of the study. Histological examinations showed mild structural distortions of the organs studied in the experimental group. Interpretation: The present study shows that alloxan injection caused a significant increase in plasma glucose levels in rats in comparison with the control rats. Persistent hyperglycaemia resulted in a number of biochemical and pathological changes in the diabetic rats but the control rats were normoglycaemic and showed no biochemical or pathological changes .There was a correlation between GGT and TAC, indicative of oxidative stress on the diabetic rats.

MSG (4,6,8, 10 and 12g/kgbw)