ABSTRACT:The aim of this in vitro study was to evaluate by spectrophotometry the influence of the incremental technique and progressive light curing in the microleakage of Class V cavities. Forty samples were prepared with class V cylindrical cavities on the buccal root surface of bovine incisive teeth and filled with composite resin (Z250). The samples were divided into four groups: I: cavity was bulk filled and the composite was light cured for 40 seconds; Group II: cavity was bulk filled and a "soft-start" polymerization was used; Group III: cavity was filled with the incremental technique in two coats and light cured for 40 seconds; Group IV: cavity was filled with the incremental technique in two coats and light cured with "soft-start" polymerization. After the restoration, the specimens were thermally stressed for 3,000 cycles in bath at 5 ± 2°C and 55 ± 2°C, protected with nail enamel, colored with 2% methylene blue and cut into sections. These sections were triturated and the dye was recovered with PA ethanol and the supernatant was evaluated. The data were submitted to ANOVA and the results showed the following averages: bulk filled and conventional photopolymerization (I) 0.06075 µg/ml; bulk filled and progressive photopolymerization (II) 0.04030 µg/ml; incremental insertion and conventional photopolymerization (III) 0.04648 µg/ml; incremental insertion and progressive photopolymerization (IV) 0.04339 µg/ml. No significant statistic differences were observed among the mean values. The Degulux "soft-start" equipment probably emits too high initial light intensity to promote progressive photopolymerization. DESCRIPTORS: Dental leakage; Dentin-bonding agents; Composite resins. RESUMO:O objetivo deste estudo in vitro foi avaliar através de espectrofotometria a influência das técnicas de inserção incremental e de fotopolimerização progressiva na microinfiltração de cavidades Classe V. Foram preparadas 40 cavidades cilíndricas na superfície radicular vestibular de incisivos bovinos e preenchidas com resina composta (Z250). Os espécimes foram divididos em quatro grupos: I: a resina foi inserida em camada única e fotopolimerizada por 40 segundos; II: a resina foi inserida em camada única e fotopolimerizada progressivamente com um aparelho "soft-start"; III: a resina foi inserida em duas camadas e fotopolimerizada por 40 segundos; IV: a resina foi inserida em duas camadas e fotopolimerizada progressivamente. Em seguida os espécimes foram submetidos a 3.000 ciclos térmicos a 5 ± 2°C e 55 ± 2°C, preparados, corados com azul de metileno a 2%, seccionados, triturados e avaliados em espectrofotometria para se quantificar o corante infiltrado. Os dados foram submetidos a ANOVA. Os resultados demonstraram as seguintes médias: inserção única e fotopolimerização convencional (I): 0,06075 µg/ml; inserção única e fotopolimerização progressiva (II): 0,04030 µg/ml; inserção incremental e fotopolimerização convencional (III): 0,04648 µg/ml; inserção incremental e fotopolimerização progressiva (IV): 0,04339 µg/ml. Não foram e...

Ao meu pai, Yoichi, Cirurgião-Dentista, por ser ao mesmo tempo meu pai, professor, incentivador e orientador da minha vida profissional. A quem devo grande parte de todas as minhas conquistas. Pela sua sede de conhecimento e amor à profissão, mesmo após quase 5 décadas de atuação. À minha mãe, Anna, por ser minha mãe, sempre fazendo de tudo por mim até hoje. Desde a infância sempre investindo na minha formação intelectual e pessoal. A cada dia que amadureço, percebo o quanto você fez por mim. A quem está por trás de todas as minhas conquistas. Aos meus irmão André e Carolina, por todas as vezes que me ajudaram quando precisei. Por todos os momentos alegres que me proporcionam até hoje.

One-bottle adhesives, both self-etching as etch-and-rinse, are complex mixtures of hydrophilic and hydrophobic monomers resin combinations dissolved in water / solvent. Although the inclusion of hydrophilic monomers has achieved high immediate adhesion forces, studies have shown that dentin-resin interface have become much weaker over time. In this study, bond strength of four adhesive systems was evaluated bymicrotensile test and quantitative analyze ofthe hybrid layer was obtained by micro-Raman spectroscopy. For microtensile test, the factors of variation were 2: the type of adhesive (AdperScotchbondMulti-Purpose, AdperSingle Bond 2, Adper SE Plus, AdperEasy One), and the period of storage at 3 levels (1 day, 30 days, 180 days), totaling 12 levels of variation. The experimental units were made on 120 dentin surfaces of intact humanunerupted third molars (n = 10). The parametric analysis of variance ANOVA and multiple comparison Games Howell was used for statistical analysis. For micro-Raman spectroscopy were used three teeth for each adhesive system, with a total of 12 teeth (n = 3). Raman spectra of the samples were recorded on a Micro-Raman spectrometer RENISHAW invia with 785 nm excitation (laser diode, RENISHAW) coupled to a microscope LEICA DM2500M with motorized stage (x, y, z) RENISHAW MS 20 EncodedStage with lateral and axial resolutionof 100 nm and 1 microns. Etch-and-rinse adhesives ScotchbondMulti-Purposeand SingleBond of this study showed the highest bond strength values, with values within a range from 44.36 to 50.66 MPa, whereas the self-etch adhesives SE Plusand Easy One had values of 17.97 to 24.60 MPa. The adhesive that showed the highest bond strength was ScotchbondMulti-Purpose and the worst performance was fromEasy One.All adhesives showed a significant decrease in bond strength after 30 and 180 days. In micro-Raman analysis, the ScotchbondMulti-Purpose was more homogeneous and had penetration values similar to those of the adhesive Single Bond and Easy One. The SE Plus had the lowest penetration of the adhesive and the thinner hybrid layer. We conclude that evaluating 4 different types of adhesives of the same brand, there is a negative relationship between the strategies of adhesion (decreasing the number of steps and bottles) and bond strength and quality of the hybrid layer. Keywords: Adhesion. Hybrid layer.Micro-Raman. LISTA DE GRÁFICOS Gráfico 5.1-Intervalos de confiança de 95% para os grupos do fator Adesivos ...... Gráfico 5.2-Intervalos de confiança de 95% para os grupos do fator Tempo ......... Gráfico 5.3-Intervalos de confiança de 95% para os grupos da interação Adesivos x Tempo .