Background: Serine residues in extensin, a cell wall protein in plants, are glycosylated with O-galactose. Results: Genes encoding the serine O-␣-galactosyltransferase were isolated from Chlamydomonas reinhardtii and plants and were characterized. Conclusion: The serine O-␣-galactosyltransferases belong to a novel glycosyltransferase class existing only in the plant kingdom. Significance: The identification of novel glycosyltransferases contributes to elucidation of how protein glycosylation has evolved in plants.

We characterized peptidyl hydroxyproline (Hyp) O-galactosyltransferase (HGT), which is the initial enzyme in the arabinogalactan biosynthetic pathway. An in vitro assay of HGT activity was established using chemically synthesized fluorescent peptides as acceptor substrates and extracts from Arabidopsis (Arabidopsis thaliana) T87 cells as a source of crude enzyme. The galactose residue transferred to the peptide could be detected by high-performance liquid chromatography and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry analyses. HGT required a divalent cation of manganese for maximal activity and consumed UDP-D-galactose as a sugar donor. HGT exhibited an optimal pH range of pH 7.0 to 8.0 and an optimal temperature of 35°C. The favorable substrates for the activity seemed to be peptides containing two alternating imino acid residues including at least one acceptor Hyp residue, although a peptide with single Hyp residue without any other imino acids also functioned as a substrate. The results of sucrose density gradient centrifugation revealed that the cellular localization of HGT activity is identical to those of endoplasmic reticulum markers such as Sec61 and Bip, indicating that HGT is predominantly localized to the endoplasmic reticulum. To our knowledge, this is the first characterization of HGT, and the data provide evidence that arabinogalactan biosynthesis occurs in the protein transport pathway.

The antibacterial activities of 81 edible plants against the dental caries pathogen Streptococcus mutans were investigated. The fresh vegetative crude extracts were subjected to the paper disc method. Furthermore, in order to fractionate the active component, hexane, ethyl acetate and methanol extracts from freeze-dried samples were also examined. Antibacterial activities were positive in 17 samples, including cinnamon and Japanese ginger. Among these, the stabilities of the active components against heat treatment or storage at 4 ℃ for one week were also investigated. Following these treatments, the activities of balsam pear and garlic extracts were lost, while the active components in ginger, Japanese ginger, clove and cinnamon appeared. Samples of the genus Zingiberaceae, including Japanese ginger and ginger, contained abundant and stable antibacterial components acting against S. mutans.