This study investigates the production of bioethanol from cassava peels using the inherent isolated microorganisms. Cassava peels were collected from cassava processing unit in selected towns in Ekiti State. Microorganisms were isolated from the cassava peels by using pour plate method; the isolates were identified by different biochemical tests and molecular characterization. The bacterial isolates include, Sporosarcina terrae, Bacillus cereus, Proteus mirabilis and Pseudomonas taiwanensis; while the fungal isolates include yeast (Wickerhamomyces rabaulensis), Rhizopus and Aspergillus niger. The entire bacterial isolates and A. niger tested positive to degradative test, while only W. rabaulensis tested positive to fermentation test. Four bacterial species were detected using 16S rDNA nucleotide sequence. High yields of the bioethanol was obtained using the combination of different inoculants, however the combination of B. cereus and W. rabaulensis produced the highest yield of 19.3 g/cm3 at a concentration of 38.6% and 17.7 g/cm3 at a concentration of 34.4% from 100g of cassava peels when distilled at 14 days and 21 days respectively. Yields of 562.31g/cm3 (67.0%) and 481.9g/cm3 (65%) of bioethanol were obtained from 5 kilograms (Kg) of cassava peels with a combination of B. cereus and W. rabaulensis and a combination of P. taiwanensis and W. rabaulensis respectively. The results of this study confirmed that bioethanol can be produced from cassava peels using the inherent isolated microorganisms as it is simple to produce, environmental friendly. and reliable therefore, healthy environment, wealth and energy generation from wastes (cassava peels) is assured in Ekiti State.
The use of purses and pouches were recommended to prevent protracted body contact with currency notes in order to abate microbial contamination of the notes, however many currency users do not comply with the use of purses, and many pouches where these notes are kept are usually dirty. It is therefore imperative to look for a way of disinfecting the notes and the environment in which it is kept. Camphor has been discovered as an effective insecticide and has been used by people to safeguard fabrics without adverse health effect. It is instructive to investigate the possibility of using the substance as antimicrobial agent for currency notes. One hundred and twenty- eight (128) samples of currency notes containing different denominations were collected at random from different sources in Ado- Ekiti metropolis. Sixty-four (64) samples were treated with camphor for six hours; the other sixty-four samples were left untreated. Isolation of microorganisms was carried out using pour plate method, microbial loads and antimicrobial activity of camphor on the samples was determined. Isolates from the samples included Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Enterobacter aerogenes, Aspergillus flavus, Mucor, Rhizopus nigrican and Scopulariopsis, but E. aerogenes, Rhizopus nigrican and Scopolariopsis were not isolated on the ₦500 and ₦1000 notes of the treated samples. The microbial loads of untreated currency notes ranged from 1.70 ×102 – 2.4× 102 cfu/ml while that of treated samples ranged from 1.00 × 102 – 1.2 × 102 cfu/ml. The reduction in the number of microorganisms and the microbial loads of the treated samples is evidence that camphor could be used as an antimicrobial agent on currency notes.
This study investigates phytochemical composition and the antimicrobial activities of aqueous and ethanol extracts of soursop (Annona muricata) leaves against some selected bacterial pathogens. Susceptibility effects of these extracts were determined by disc diffusion method against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus pyogenes and Staphylococcus aureus. The results showed that the efficacy of the aqueous extracts was more pronounced against the test bacterial, especially P. aeruginosa at minimum inhibitory concentration (MIC) of 0.2 mg/mL. The ethanol extract was more pronounced again S. pyogenes with diameter of zones of inhibition ranging from 15.00 – 32.00 mm at concentrations of 0.2 – 0.8 mg/mL respectively. The pathogens were susceptible to the aqueous extracts of the leave at concentrations between 0.4 – 0.8 mg/mL. Phytochemical compounds such as tannins, saponins, terpenoids, alkaloids, glycosides, and phenols were detected in A. muricata leaf extract. The significant antimicrobial properties of the leaf extract could be attributed to the presence of these bioactive compounds. Thus, this investigation proves to an extent that the Soursop leaf extracts when used against microorganisms, has sufficient antimicrobial property.
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