Detection of microsporidia in clinical specimens has relied on electron microscopy, histology, or staining. This article describes further alterations to the modified trichrome staining method which make it easier to identify microsporidial spores. The changes are a decrease in the phosphotungstic acid level and the substitution of a colorfast counterstain, aniline blue, for the fast green of the original stain. The modified stain provides good contrast between microsporidial spores and background material including human and fungal cells. Stool specimens from 139 human immunodeficiency virus-seropositive patients revealed that 5 patients were infected with Enterocytozoon bieneusi and 6 patients had larger spores. Thin-section electron microscopy of the larger spores showed a structure consistent with that of either Encephalitozoon or Septata species. Three of the patients with Encephalitozoonor Septata-like species had disseminated infection, with spores detected in nasopharyngeal aspirates and urine samples.

Three types of alimentary canals (=midgut) occur in the Annelida and non-trilobite Arthropoda: 1) a sagittal tube with metamerically paired diverticula related to the number of somites; 2) a tube that is constricted slightly between somites; and 3) a simple tubular gut that may taper slightly backwards to the anus. At least two of these three types (1 and 3) occur in the Trilobita. Pterocephalia and Olenoides share the first type with the probable sister taxon to the Trilobita, Naraoia (Nectaspida), and this is probably the plesiomorphic condition for the class. Varying feeding habits may well have made this character homoplastic within each of these groups. The preservation of parts of the alimentary tract in specimens of Upper Cambrian Pterocephalia n. sp. (McKay Group, British Columbia) was probably a function of taphonomic and/or very early diagenetic changes that resulted from the type of food preferred by that trilobite. Other trilobites from the same beds do not have their soft parts preserved. The alimentary structures are preserved in a different fashion from, apparently unattached to, and an order of magnitude larger than genal caeca that occur in this taxon. Thus, genal caeca are regarded as imprints of circulatory rather than alimentary structures.Energy dispersive analysis of a fragment of preserved alimentary tract of Pterocephalia n. sp. showed the presence of Ca, Si, Al, Fe, P, K, Na, and Cl. These alimentary tracts are composed of a complex mixture of minerals that probably includes clays, detrital quartz, carbonates, phosphates, and oxides or hydroxides. The structure of these dark fillings is microcrystalline. The presence of detrital minerals as part of this mixture would suggest that this trilobite was a deposit feeder.

An outbreak of acute gastroenteritis of suspected viral aetiology occurred in April 2003 in the British Royal Fleet Auxiliary ship (RFA) Argus deployed in the Northern Arabian Gulf. There were 37 cases amongst a crew of 400 personnel. Of 13 samples examined from cases amongst the crew, six enteric viruses were detected by reverse transcriptase polymerase chain reaction (RT-PCR). Five different viruses were identified including, three norovirus genotypes, a sapovirus and a rotavirus. No multiple infections were detected. A common food source was implicated in the outbreak and epidemiological analysis showed a statistically significant association with salad as the source of the outbreak, with a relative risk of 3.41 (95% confidence interval of 1.7-6.81) of eating salad on a particular date prior to the onset of symptoms. Faecal contamination of the salad at source was the most probable explanation for the diversity of viruses detected and characterized.