The Gas Liquid Chromatographic Method for the separation of sugars has been developed to provide a routine quantitative determination of the sugar components in beer and wort, and in sugar-containing brewing adjuncts. Samples for test are dried under vacuum and converted to volatile trimethylsilyl (T.M.S.) derivatives which are suitable for analysis.The peak areas of the sugars are corrected to an internal standard and the concentrations calculated by applying the appropriate detector response factors.By the use of the method, the quantitative determination pf fructose, glucose, maltose, maltotriose and maltotetraose in samples can be accomplished at a rate of 6-7 complete analyses per working day with the expenditure of comparatively little actual operator time.

A sensitive routine quantitative method has been developed which determines acetaldehyde in beer. The method is suitable for the measurement of acetaldehyde, its bisulphite addition compound and acetal, with only limited interference from other aldehydes. The examination of a representative selection of beers shows that large differences of acetaldehyde can occur in beers of a similar type. Beers which have been sweetened by the addition of priming sugar vary widely in their acetaldehyde content, many being above the 25 ppm taste threshold. The acetaldehyde content has been shown to vary with both the quantity and point of addition of sulphur dioxide when this is used as a preservative or antioxidant.

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Ion exchange chromatography has been used to provide a quantitative automatic analysis of lupolunes, humulones, humulinic add, Iso-a-acld and hulupones.The resins are extracted and chromatographed on a column of Q.A.E. Sephadex in the acetate form, using an acetic acid gradient in methanol as eluent. The absorbance of the effluent is recorded and the peaks are integrated. A comparison of peak areas with known standards gives a quantitative analysis of the hop components.

Various methods for the separation of amino acids from brewing materials and subsequent analysis by gas chromatography have been examined. Separation of the amino acids by ion exchange chromatography followed by derivatization and gas chromatography of the trifluoracetyl n-butyl or heptafluorobutyl iso-amyl esters proved the most satisfactory methods to employ.