Bioluminescence is a biochemical process occurring in many organisms. Bacterial bioluminescence has been investigated extensively that lead to many applications of such knowledge. Quorum sensing in the bioluminescent bacteria is a chemical signal process to recognize the strength of its own population to start luminescence in harmony. There is a mechanism in these bacteria to also recognize inter-species strength. When there is a higher number of these bacteria, the possibility and frequency of cell-cell physical contact will be high. In this study, the physical proximity was artificially enhanced between cells and the effect on luminescence in the concentrated cells in the normal culture medium and in the presence of other non-bacterial cell-free supernatants was investigated. The role of such physical contact in the quorum sensing in the bioluminescence is not known. Increase in the luminescence of V. fischeri when concentrated shows that the presence of physical proximity facilitates the quorum sensing for their bioluminescence.

Abstract-UI testing is a process of testing a software application or functionality of GUI. It is defined as an interface between user and software which provides an easy way to interact with the system. GUI plays an important role in software engineering. GUI-based application requires that a test case consists of sequences of user actions/events to be executed. Selenium is an open-source tool for testing GUI Application by executing test cases whether the GUI Application is working properly or not. In this, we present test script repair technique to repair test cases. Test script repair technique uses reverse engineering process for creating the test script. Test script repair consists of three stages; they are Ripping, Mapping and Repairing. In ripping stage, there are two relationships for representing event interaction of GUI Application. During ripping we know the location of each widget. In mapping stage,original GUI events are mapped to an event-flow graph (EFG). In repairing stage, Event flow graph uses repairing transformations and human input to modified script to repair the test cases, and synthesizes a new "repaired" test script. During this process, test script repair uses GUI objects for yielding a final test script that can be executed using selenium tool to validate the GUI Application. An experiment using selenium tool to test, test cases suggests that it is effective in that unusable test scripts are repaired. Annotations significantly reduced the human cost to repair test cases.