Non-destructive techniques for the demonstration of chemical changes in the rhizosphere of soil-grown plants are described. The following processes are demonstrated: FeIII reduction indicated by the formation of a red coloured complex between FeII and bathophenanthroline-disulfonate (BPDS) in an agar medium; MnIV reduction indicated by the decolourization of filter paper impregnated with Mn oxide; complexation of A1 by the decolourization of polyacrylamide gel or agar containing A1 and aluminon; and the activity of acid phosphatase indicated by the formation of a red complex on filter paper containing 1-naphthyl phosphate as substrate and Fast Red TR as an indicator.
Chemical changes in the rhizosphere of soil-grown plants are d e m o n s t r a t e d by non-destructive techniques based on colour reactions. The following examples are given: FeIII reduction in the rhizosphere of a Hakea species, MnIV reduction in the rhizosphere of chickpea, complexation of A1 in the rhizosphere of Norway spruce, and the activity of acid phosphatase in the rhizosphere of maize.
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