To extend the practical applications of bulk metallic glasses, the preparation of metallic glass coatings on various substrates becomes an important research issue. Among the interfacial properties of the coatings, the adhesion between films and substrates is the most crucial. In this study, amorphous Zr 61 Al 7?5 Ni 10 Cu 17?5 Si 4 (ZrAlNiCuSi) thin films were deposited on SUS 304 stainless steel at various sputtering powers by dc sputtering. According to the scratch tests, the introduction of Cr and Ti buffer layers effectively improves the adhesion between the amorphous thin films and substrate without changing the surface properties, such as roughness and morphology. The antimicrobial results show that the biological activities of these microbes, except Acinetobacter baumannii, are effectively suppressed during the test period.