Basil (Ocimum sp.) is generally known as "king of herbs" due to its pharmaceutical and culinary properties. Even though Ocimum sp. are considered to have fast germination velocity index (GVI), short mean germination time (MGT) and increased seedling vigor index (SVI) values, their experimental monitoring is usually done with specialized software's that are expensive. Low cost scientific solutions are keen on open source software germination protocols. For observing the accuracy of open source, C.A.D. softwares were used for Ocimum sp. seeds which were submitted to high frequency pulsatile electromagnetic fields (300 impulses/3 peak penetrance -293 W) and ultrasound pulsatile fields (1/2 pulses by 0.5 W/cm 2 ). All sequence images where taken using a positive/negative photo filter enhancing the differences in seed development. At the end of the experiment, all images where transformed into vector formats (dwg). On the dwg extension, selective free plug-ins such as Face Centroid and Area Properties helped to collect data like seed development on X/Y scale, area, perimeter, no. of germinated seeds, length of seedling root, hypocotyl length. Mean germination time (MGT) was in agreement with the accepted theory of seed germination that enounced a diametric position between values of GVI and MGT, meaning that all the experimental variants with high values of GVI recorded low values of MGT. All the experimental variants influenced by physical stimulants recorded improved contents of therapeutical terpenes.
The study illustrates the influence of high frequency pulsatile electromagnetic fields and ultrasound pulsatile fields on Mentha × piperita L. seed germination and the quality of its essential oil. The physiological role of the above mentioned experimental factors was considered to be a catalyticall base point, improving germination percent, SVI (seedling vigor index), GVI (germination velocity index). All the biometric aspects of the germination process (seed area, seed perimeter, seed development on x and y radius, radicele length, hypocotyl length) where determined using open free software, consolidating the general idea that scientific communities can improve and perfect open source projects. High frequency pulsatile electromagnetic fields (91.75%) and ultrasound pulsatile fields (64.75%) experimental variants gave higher germination percent compared to control (47.00%). Following the main terpenes determination, the same experimental variants obtained high accumulations of menthol, eugenol, thymol, eucalyptol, linalool and other components. These aspects can be scientifically sustained by the seedling vigor index marks obtained at high frequency pulsatile electromagnetic fields (1985.47) and ultrasound pulsatile fields (1480.09), creating the general premises for better development stages in the nursery sector. Raised accumulation of main therapeutical terpenes in Mentha × piperita L. must be supervised in further studies, when microscopically imagery of glandular trichomes and their density may lead to more profound conclusions.
Basil (Ocimum sp.) is generally known as "king of herbs" due to its pharmaceutical and culinary properties. Even though Ocimum sp. are considered to have fast germination velocity index (GVI), short mean germination time (MGT) and increased seedling vigor index (SVI) values, their experimental monitoring is usually done with specialized software's that are expensive. Low cost scientific solutions are keen on open source software germination protocols. For observing the accuracy of open source, C.A.D. softwares were used for Ocimum sp. seeds which were submitted to high frequency pulsatile electromagnetic fields (300 impulses/3 peak penetrance -293 W) and ultrasound pulsatile fields (1/2 pulses by 0.5 W/cm 2 ). All sequence images where taken using a positive/negative photo filter enhancing the differences in seed development. At the end of the experiment, all images where transformed into vector formats (dwg). On the dwg extension, selective free plug-ins such as Face Centroid and Area Properties helped to collect data like seed development on X/Y scale, area, perimeter, no. of germinated seeds, length of seedling root, hypocotyl length. Mean germination time (MGT) was in agreement with the accepted theory of seed germination that enounced a diametric position between values of GVI and MGT, meaning that all the experimental variants with high values of GVI recorded low values of MGT. All the experimental variants influenced by physical stimulants recorded improved contents of therapeutical terpenes.
The study illustrates the influence of high frequency pulsatile electromagnetic fields and ultrasound pulsatile fields on Mentha × piperita L. seed germination and the quality of its essential oil. The physiological role of the above mentioned experimental factors was considered to be a catalyticall base point, improving germination percent, SVI (seedling vigor index), GVI (germination velocity index). All the biometric aspects of the germination process (seed area, seed perimeter, seed development on x and y radius, radicele length, hypocotyl length) where determined using open free software, consolidating the general idea that scientific communities can improve and perfect open source projects. High frequency pulsatile electromagnetic fields (91.75%) and ultrasound pulsatile fields (64.75%) experimental variants gave higher germination percent compared to control (47.00%). Following the main terpenes determination, the same experimental variants obtained high accumulations of menthol, eugenol, thymol, eucalyptol, linalool and other components. These aspects can be scientifically sustained by the seedling vigor index marks obtained at high frequency pulsatile electromagnetic fields (1985.47) and ultrasound pulsatile fields (1480.09), creating the general premises for better development stages in the nursery sector. Raised accumulation of main therapeutical terpenes in Mentha × piperita L. must be supervised in further studies, when microscopically imagery of glandular trichomes and their density may lead to more profound conclusions.
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