The in vitro cultures of apple rootstock MM 106 produced the highest fresh mass (FM) when 0.1 mM B was included in the culture medium. By increasing B concentration of the culture medium from 0.1 to 6.0 mM, FM and contents of B, P, Ca, and Mg in explants increased, whereas K, Fe, Mn, and Zn contents decreased. SPAD units of leaves characterizing chlorophyll contents declined as B concentration of the culture medium increased from 0.1 to 6.0 mM.

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The effects of five different N-sources (KNO 3 + NH 4 NO 3 = control, KNO 3 , NH 4 NO 3 , NH 4 H 2 PO 4 , L-alanine) on growth, nutritional status, chlorophyll (Chl) content, and photosynthetic parameters of the apple rootstock MM 106 shoots cultured in vitro were investigated. In comparison to all the other treatments, control explants grown on a MS medium containing KNO 3 + NH 4 NO 3 had the highest fresh mass, Chl content, net photosynthetic rate, transpiration rate, and stomatal conductance.