Effects of different N-sources on growth, nutritional status, chlorophyll content, and photosynthetic parameters of shoots of the apple rootstock MM 106 cultured in vitro

Sotiropoulos, Thomas; Mouhtaridou, G.N.; Thomidis, T.; Tsirakoglou, V.; Dimassi, K.; Therios, Ioannis

doi:10.1007/s10535-005-7299-6

Cited by 14 publications

(8 citation statements)

References 14 publications

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“…46 Another reason is ammonium application reduces the uptake of potassium, which affects stomatal function. 47 In consistent with our results, Sotiropoulos et al 48 reported that ammonium-N reduced chlorophyll content in apple. This lower chlorophyll content might be due to the accumulation of ammonium in the leaf, which increases the sensitivity to ethylene which resulted in chlorophyll loss.…”

Section: Discussionsupporting

confidence: 92%

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Nitrogen preference and genetic variation of cotton genotypes for nitrogen use efficiency

et al. 2020

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BACKGROUND Although nitrogen (N) availability is a major determinant of cotton production, little is known about the importance of plants' preference for ammonium versus nitrate for better growth and nitrogen use efficiency (NUE). We aimed to assess the growth, physiology, and NUE of contrasting N‐efficient cotton genotypes (Z‐1017, N‐efficient and GD‐89, N‐inefficient) supplied with low and high concentrations of ammonium‐ and nitrate‐N. RESULTS The results revealed that ammonium fed plants showed poor root growth, lower dry biomass, N content, leaf chlorophyll and gas exchange than those under nitrate irrespective of the concentration. However, the highest N uptake and utilization efficiency were obtained with nitrate fed plants, which also resulted in the highest dry biomass, N content, leaf chlorophyll and gas exchange as well as root growth. The results further confirmed that N‐efficient (Z‐1017) genotype performed better under both N sources, showing more flexibility to contrasting N condition by increasing growth and NUE in either source of N. Moreover, multivariate analysis showed a strong relationship of root morphological traits with N utilization efficiency, suggesting the physiological importance of roots over shoots in response to low nitrate concentration. CONCLUSION Thus, it was confirmed that nitrate‐N is superior to ammonium‐N and the use of nitrate and N‐efficient genotype is the best option for optimum cotton growth and NUE. Further, field evaluation is required to confirm the hypothesis that nitrate is a preferred N source for better cotton production and NUE. © 2020 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 92%

“…Another reason is ammonium application reduces the uptake of potassium, which affects stomatal function . In consistent with our results, Sotiropoulos et al . reported that ammonium‐N reduced chlorophyll content in apple.…”

Section: Discussionsupporting

confidence: 92%

Nitrogen preference and genetic variation of cotton genotypes for nitrogen use efficiency

et al. 2020

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“…This type of enhanced percentage of embryoid induction was noticed in cotton by the supplementation of NH 4 NO 3 and MgSO 4 (Ganesan and Jayabalan 2004). Like our results, in apple rootstock, addition of N-sources like KNO 3 and MH 4 NO 3 along with MS medium showed best response for the in vitro culture studies (Sotiropoulos et al 2005).…”

Section: Somatic Embryo Induction and Maturationsupporting

confidence: 87%

Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture

Ganesan¹,

Chandrasekar²,

Kumari³

et al. 2007

Biologia plant.

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A simple and reliable protocol for regeneration of okra through somatic embryogenesis from suspension cultures has been developed. Embryogenic callus was obtained from hypocotyl explants cultured on media with Murashige and Skoog (MS) salts, Gamborg (B5) vitamins, 2.0 mg dm -3 2,4-dichlorophenoxyacetic acid (2,4-D), 1.0 mg dm -3 naphthaleneacetic acid (NAA), 25 mg dm -3 polyvinylpyrrolidone and 30 g dm -3 sucrose. More number and high frequency of healthy embryoids appeared individually in suspension culture containing MS salts, B5 vitamins, 2.0 mg dm -3 2,4-D and 1.0 mg dm -3 kinetin. Formation of cell clusters from the single cells was clearly noticed during ontogeny. Matured embryos at the cotyledonary stage were transferred to agar solidified medium for germination. The best conversion of embrya into plantlets (67.3 %) was recorded on media with half strength MS salts, B5 vitamins, 0.2 mg dm -3 benzylaminopurine (BAP) and 0.2 mg dm -3 gibberellic acid (GA 3 ). The plantlets were transferred to soil and hardened in the plastic pots. After proper acclimatization, the plantlets regenerated through somatic embryogenesis were compared to seed grown plants to observe any variation.

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“…1). Sotiropoulos et al (2005) also reported that apple rootstock had a higher fresh mass with the supply of NH 4 NO 3 as the N source. The finding that biomass was limited by a sole NH 4…”

Section: Discussionmentioning

confidence: 90%

NO3 −/NH4 + ratios affect plant growth, chlorophyll content, respiration rate, and morphological structure in Malus hupehensis seedlings

Zhi

Zhao

et al. 2015

J. For. Res.

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Malus hupehensis (Pamp.) Rehd. is a widely cultivated rootstock in China. We studied the effect of three NO 3 -/NH 4 ? ratios (100/0, 50/50, and 0/100, molar basis) at total nitrogen (N) concentration of 8 mmoL L -1 in a nutrient solution on M. hupehensis seedlings. Plant biomass, NO 3 -and NH 4 ? concentration, chlorophyll content, respiratory rate, and cellular structure were investigated. M. hupehensis seedlings at the NO 3 -/NH 4 ? ratio of 50/50 had the highest level of fresh weight, dry weight, shoot length, and chlorophyll (a, b, and a ? b) content, but the lowest respiration rate in the leaves and roots. In addition, thickness and numbers of palisade and spongy tissue cells of the leaves were greater with this treatment than with other treatments. At the NO 3 -/NH 4 ? ratio of 100/0, the leaves and roots had higher NO 3 -concentration and lower NH 4 ? concentration. However, the opposite trend occurred at the NO 3 -/NH 4 ? ratio of 0/100. Chlorophyll (a, b, and a ? b) content was lowest at the NO 3 -/ NH 4 ? ratio of 100/0 than at the other ratios. At the NO 3 -/ NH 4 ? ratio of 0/100, oxygen (O 2 ) consumption increased in the leaves and roots, and irregular epidermis and cortex cells were observed in the root apical meristematic and mature region. Our results indicated that the NO 3 -/NH 4 ? ratio at 50/50 was suitable for growth of M. hupehensis seedling to achieve the highest biomass production and efficiency.

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Effects of different N-sources on growth, nutritional status, chlorophyll content, and photosynthetic parameters of shoots of the apple rootstock MM 106 cultured in vitro

Cited by 14 publications

References 14 publications

Nitrogen preference and genetic variation of cotton genotypes for nitrogen use efficiency

Nitrogen preference and genetic variation of cotton genotypes for nitrogen use efficiency

Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture

NO3 −/NH4 + ratios affect plant growth, chlorophyll content, respiration rate, and morphological structure in Malus hupehensis seedlings

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