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In a pot experiment, the effects of the bacterium strain Micrococcus roseus, native soil arbuscular mycorrhizal fungi (AMF) and the fungus Glomus mosseae on the growth, P, N, Fe, Mn, Zn and Cd uptake of maize in a soil polluted with Cd were investigated. A three-factor experiment was set up in a randomized complete design with three replicates of each treatment. The factors in the experiment were as follows: 1) AMF with two levels, G 1 (native AMF) and G 2 (G. mosseae + G 1 ); 2) bacterium promoting plant growth with two levels, B 0 (no inoculation) and B 1 (inoculation with M. roseus); and 3) cadmium with three levels (0, 100 and 200 mg Cd kg -1 soil). G 2 significantly increased root colonization, plant biomass, shoot nutrients and Cd uptake of plants in comparison with G 1 in Cd polluted conditions. The single presence of AMF contributed to the stabilization of Cd in roots and soil. Shoot and root dry weights, and shoot nutrients uptake of plants co-inoculated with bacterium and AMF were higher than mycorrhizal plants in the soil polluted with Cd. Plants co-inoculated with bacterium and G 2 had higher amount of shoot Cd uptake, root Cd uptake, Cd phytoextraction, translocation, and uptake efficiencies under both Cd concentrations than only mycorrhizal plants. The results showed that, most of the Cd was sequestered in roots of plants co-inoculated with bacterium and native AMF in the soil with 100 and 200 mg Cd kg -1 . Additional key words: cadmium; Glomus mosseae; Micrococcus roseus; native AMF; phytoremediation; Zea mays L. ResumenInfluencia de los hongos micorrícicos arbusculares y de una bacteria promotora del crecimiento en la absorción de Cd y el crecimiento del maíz en suelos contaminados con Cd Se investigaron, en un experimento en macetas, los efectos de la bacteria Micrococcus roseus, hongos micorrícicos arbusculares (AMF) nativos del suelo y el hongo Glomus mosseae en el crecimiento, así como en la absorción de P, N, Fe, Mn, Zn y Cd por el maíz en un suelo contaminado con Cd. Se estableció un experimento trifactorial en un diseño completamente al azar con tres repeticiones por tratamiento. Los factores fueron: 1) AMF con dos niveles, G 1 (AMF nativos) y G 2 (G. mosseae + G 1 ), 2) bacterias promotoras del crecimiento de las plantas con dos niveles, B 0 (sin inoculación) y B 1 (inoculación con M. roseus); y 3) cadmio a tres niveles (0, 100 y 200 mg Cd kg -1 suelo). En condiciones de contaminación con Cd, G 2 aumentó significativamente, comparado con G 1 , la colonización de las raíces, la biomasa vegetal, y la absorción de nutrientes y Cd en los brotes de las plantas. La sola presencia de AMF contribuyó a la estabilización de Cd en las raíces y el suelo. En el suelo contaminado con Cd, el peso seco de los brotes y las raíces, y la absorción de los nutrientes de los brotes en las plantas coinoculadas con la bacteria y los AMF fueron mayores que en las plantas micorrizadas. Las plantas coinoculadas con la bacteria y G 2 presentaron mayor cantidad de absorción de Cd en los brotes y raíces, fitoe...

A b s t r a c t. To investigate the effect of amended substrates on cadmium uptake by one-year old poplar rooted cuttings a pot culture was carried out. Pots were filled with three substrates. Four treatments of Cd supply including were organized. The results showed that higher biomass productions in substrates A and B compare to substrate C, led to an increase total Cd uptake two times more than that in substrate C, at 150 mg kg -1 concentration. Meanwhile maximum total uptake occurred in substrate B at 100 mg kg -1 concentration. Using synthetic chelators such as ethylenediaminetetraacetic acid in order to achieve high removal rate led to increased environmental impacts while they are not expected when such environmental friendly approaches are applied.K e y w o r d s: soil, cadmium, ethylenediaminetetraacetic acid, phytoextraction, substrate