A cross-sectional study was performed in Southern and Lusaka provinces of Zambia between March and September 2008 to estimate Brucella seroprevalence in cattle kept by smallholder dairy farmers (n = 185). Rose Bengal test (RBT) was used as a screening test followed by confirmation with competitive ELISA (c-ELISA). We investigated 1,323 cattle, of which 383 had a history of receiving vaccination against brucellosis and 36 had a history of abortion. Overall seroprevalence was 6.0% with areas where vaccination was practiced having low seroprevalence. Age was associated with Brucella seropositivity (P = 0.03) unlike cattle breed (P = 0.21) and sex (P = 0.32). At area level, there was a negative correlation (Corr. coeff = -0.74) between percentage of animals with brucellosis vaccination history (vaccination coverage) and level of brucellosis; percentage of animals with history of abortion (Corr. coeff. = -0.82) and brucellosis vaccination coverage. However, a positive correlation existed between brucellosis infection levels with percentage of animals having a history of abortion (Corr. coeff. = 0.72). History of vaccination against brucellosis was positively associated with a positive Brucella result on RBT (P = 0.004) whereby animals with history of vaccination against brucellosis were more likely to give a positive RBT test results (OR = 1.52). However, the results of c-ELISA were independent of history of Brucella vaccination (P = 0.149) but was positively associated with history of abortion (OR = 4.12). Our results indicate a relatively low Brucella seroprevalence in cattle from smallholder dairy farmers and that vaccination was effective in reducing cases of Brucella infections and Brucella-related abortions. Human exposure to Brucella through milk from smallholder farmers could result through milk traded on the informal market since that milk is not processed and there no quality and safety controls.

The Zambian veterinary delivery system is responding to changes in the socioeconomic environment and in the structural organisation and functions of the delivery system itself. Privatisation and liberalisation are now being actively pursued as official Government policy with free competition being encouraged. As a profession, veterinarians must safeguard their interests by, among other actions, self-regulating their activities so as to enhance their standing in society and to develop or maintain standards worthy of the profession. This will be achieved largely through the Veterinary Association of Zambia. It is also imperative for the Government to ensure that governmental policy succeeds by providing an appropriate environment and by protecting the public and animals from undesirable actions by agents of the delivery system (veterinarians and para-professionals). There is also the need to maintain harmony among these agents and to resolve any differences which develop between colleagues and subordinates. Such objectives call for laws and codes of ethics appropriate to the environment of Zambia to be established to guide practitioners, the Veterinary Association, the public and any statutory body which is established to enforce these laws and codes of conduct.