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This paper describes an efficient in vitro micropropagation of Artemisia vulgaris using shoot tip and nodal explants. Among the various growth regulators tested, MS medium and B 5 vitamins supplemented with BA (4.44 lM) and KN (2.32 lM) combination was found to yield a better response than BA (4.44-13.32 lM) or KN (0.46-13.92 lM) alone in the medium. BA and KN combinations produced a maximum of 23.3 shoots per explant with 99.8% shooting frequency. Multiple shoots raised were elongated on MS medium containing 0.44 lM BA and 1.44 lM GA 3 . Rooting was highest (98.2%) on MS medium containing 8.56 lM IAA. Rooted plantlets were successfully transferred to plastic cups containing autoclaved garden soil, farmyard soil and sand (2:1:1) for hardening. After 65 days, the plantlets were transferred to Botanical Evaluation Garden and maintained. The survival rate of plantlets varied under acclimatization. Plants looked healthy with no visually detectable phenotypic variations. This is the first report on plant regeneration via organogenesis of A. vulgaris.

An in vitro propagation system for Artemisia vulgaris L., a traditional medicinal plant, has been developed. The best organogenic response, including adventitious shoot number and elongation, was obtained when hypocotyl segments were cultured onto MS medium supplemented with 4.54 lM TDZ (N-phenyl-N¢-(1,2,3-thidiazol-yl) urea). Up to 28 shoots formed per explant for an optimal duration of exposure of 48 days. Regenerated shoots formed roots when subcultured onto a medium containing 8.56 lM IAA (indole-3-acetic acid). Healthy plantlets were transferred to a garden soil:farmyard soil:-sand (2:1:1) mixture for acclimatization, which was successful, and subsequent maturity was achieved under greenhouse conditions over a six-month period. The survival rate of the plantlets varied under acclimatization. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of A. vulgaris. This optimized protocol has been successfully employed for genetic transformation studies in A. vulgaris, which are currently underway in our laboratory.