Effect of phytohormones on micropropagation of Artemisia vulgaris L.

Kaur

Physiol Mol Biol Plants

2014

Micropropagation offers a great potential to produce millions of clonal individuals through tissue culture via induction of morphogenesis. The aim of this work was to obtain an efficient protocol for callus regeneration for Gentiana kurroo Royle. The morphogenic response of different explants (leaves, petioles, roots) varied and responded differently for regeneration according to combinations of growth regulators. The petiole explants were best responding for callus induction and subsequently for indirect and direct regeneration. The callus induction was achieved on MS basal + 1.0 mg/l benzyladenine (BA) and 3.00 mg/l naphthalene acetic acid (NAA). MS medium supplemented with 0.10 mg/l NAA and 1.0 mg/l thidiazuron (TDZ) was recorded as the best medium for indirect regeneration. However, for direct regeneration the maximum number of shoot emergence was observed on MS basal fortified with 0.10 mg/l NAA + 0.75 mg/l TDZ. Half strength MS basal supplemented with indole-3-butyric acid (IBA) 1.00 mg/l gave best response for root induction. Subsequently, the plantlets were transferred and 100 % survival rate was recorded only on autoclaved cocopeat. No morphological variations were recorded in the callus regenerated plantlets.

Section: Discussionsupporting

confidence: 90%

In vitro morphogenic response of different explants of Gentiana kurroo Royle from Western Himalayas—an endangered medicinal plant

Kaur

Physiol Mol Biol Plants

2014

Physiol Mol Biol Plants

“…BAP has been described as a beneficial growth regulator for induction of shoots in other Artemisa species (Sujatha and Ranjitha Kumari 2007; Umer Sharief and Jagadish Chandra 1991). In Artemisia vulgaris it has been observed that shooting response was increased when BAP (4.44 lM) and Kn (2.32 lM) combination rather than BAP (4.44-13.32 lM) or Kn (0.46-13.92 lM) (Sujatha and Ranjitha Kumari 2007). In the present study, MS medium supplemented with BAP in combination with 2-iP yielded optimal shoot regeneration.…”

Section: Induction Of Multiple Shoots From Callussupporting

confidence: 49%

Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

Shinde

Sebastian

Jain

et al. 2016

A reliable protocol has been established for in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood), a valuable medicinal plant from India. A highly proliferating organogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 2.5 lM IAA when nodal explants were cultured on MS medium supplemented with various growth regulators. Further, highest regeneration frequency (83.3 %) of adventitious shoots was observed, when the callus was subcultured on MS medium supplemented with 6-benzylaminopurine (BAP; 2.5 lM) along with 7.5 lM 2-isopentenyl adenine (2-iP). An optimal of 10.16 ± 2.24 shoots were regenerated on medium supplemented with 2.5 lM BAP ? 7.5 lM 2-iP. Quarter strength MS medium supplemented with 10 lM IBA was effective for rooting of the shoots. Ex-vitro plants were normal and were established successfully. Cytological and molecular marker studies showed that regenerated plants showed genetic stability in micro-propagated plants.

Acta Biologica Cracoviensia Series Botanica

“…This cytokinin plays a significant role in shoot formation as it overcomes apical dominance and promotes axillary bud development (Xu, 2008). BA has been commonly used to induce shoot organogenesis in many plants, including species of the Asteraceae (Orlikowska et al, 1999;Sujata and Ranjitha Kumari, 2007;Chakrabarty and Datta, 2008). Our results confirm the findings of an earlier study comparing the effectiveness of BA, KN and ZEA, in which BA was found to be the most and zeatin the least effective (Shiva Pakash et al, 1994).…”

Section: Resultsmentioning

confidence: 99%

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Trejgell¹,

Tretyn²

2011

This paper is the first published report describing micropropagation of Carlina onopordifolia, using shoot tip and hypocotyl explants. The explants were excised from 10-day-old seedlings and transferred to proliferation medium supplemented with 6-benzylaminopurine (BA; 1.0 or 3.0 mg l -1 ), kinetin (Kn; 1.0 or 3.0 mg l -1 ) or zeatin (ZEA; 1.0 or 3.0 mg l -1 ) in combination with naphthaleneacetic acid (NAA; 0.1 mg l -1 ). The shoot tips were significantly better than hypocotyls as initial material for shoot regeneration. For shoot multiplication, MS medium supplemented with BA proved superior to the other cytokinins tested. Medium supplemented with 1.0 mg l -1 BA gave the highest shoot propagation frequency (66.9%) and number of shoots per explant (2.5). Single shoots were separated from each other and rooted on MS supplemented with IBA for the whole period of culture, with longor short-pulse IBA application. The highest rooting frequency (84.8%) and root number (18.8) were for shortpulse (1 min) 1000 mg l -1 IBA solution. The higher IBA concentration stimulated callus formation and the development of short roots. The shoots were transferred to MS medium without growth regulators. Survival was highest (54.4%) for the plants from the short-pulse 100 mg l -1 IBA treatment. After 8 weeks of acclimatization the plantlets were removed to field conditions and grew normally.K Ke ey y w wo or rd ds s: : Carlina onopordifolia, micropropagation, cytokinins, rooting, auxin.