Ginger (Zingiber officinale) belongs to Zingiberaceae family is one of the famous spices all over the world. It is a perennial creeping plant with long leaves, yellow green flowers and thick tuberous rhizome. Silver nanoparticles’ potential uses in Green Chemistry have received attention. The current study focuses on the rapid biological production of silver nanoparticles using different plant materials and characterizations using UV-visible spectrophotometry, IR, SEM, and HPLC investigations. Within 15 minutes of adding sodium carbonate, an aqueous extract of dried Zingiber officinalis rhizome reduces silver nitrate. No further reduction and stabilizing chemicals are required for the entire process, showing a green synthesis. Escherichia coli and Staphylococcus aureus use biosynthesized “Zinger-AgNps” as a competent antibacterial agent. Zinger-AgNps were evaluated as its catalytic capability to reduce the model pollutant methylene blue. Both of them are displayed 2, 2-diphenyl-1-picrylhydrazyl-specific free radical scavenging activity (DPPH). Silver biological material nanoparticles are created by utilizing less hazardous and nontoxic reduction agents, such as ascorbic acid and sodium citrate. The antioxidant and lipid peroxidation inhibition properties of ginger prevent peroxidative damage, indicating the benefits of ginger in the prevention of microbial food spoilage, free-radical-induced damage and rancidity. The sodium citrate aggregation for silver nanoparticles, firm surface contact, and synthesized silver nanoparticles are used to study antibacterial efficacy and antioxidant activity.

In the title compound, C23H24F2O3, the central pyran ring has a flat-boat conformation, whereas the two fused cyclo­hexenone rings adopt envelope conformations, with the C atom bearing the dimethyl substituent being the flap atom in each case. The pyran ring mean plane and the di­fluoro­phenyl ring are almost normal to each other, making a dihedral angle of 87.55 (4)°. In the crystal, mol­ecules are linked by pairs of C—H⋯O hydrogen bonds, forming inversion dimers with an R 2 2(8) ring motif. The F atom at position 2 on the di­fluoro­phenyl ring is disordered over the 2- and 6-positions, and has a refined occupancy ratio of 0.932 (3):0.068 (3).

Background The aim of the present method is to develop and validate a specific, sensitive, precise, and accurate liquid chromatography-mass spectrometry (LC-MS) method for the estimation of the phenyl vinyl sulfone in the eletriptan hydrobromide. The effective separation of the phenyl vinyl sulfone was achieved by the Symmetry C18 (50 × 4.6 mm, 3.5 μm) column and a mobile phase composition of 0.1%v/v ammonia buffer to methanol (5:95 v/v), using 0.45 ml/min flow rate and 20 μl of injection volume, with methanol used as diluent. The phenyl vinyl sulfone was monitored on atomic pressure chemical ionization mode mass spectrometer with positive polarity mode. Results The retention time of phenyl vinyl sulfone was found at 2.13 min. The limit of detection (LOD) and limit of quantification (LOQ) were observed at 1.43 ppm and 4.77 ppm concentration respectively; the linear range was found in the concentration ranges from 4.77 to 27.00 ppm with regression coefficient of 0.9990 and accuracy in the range of 97.50–102.10%. The percentage relative standard deviation (% RSD) for six replicates said to be injections were less than 10%. Conclusion The proposed method was validated successfully as per ICH guidelines. Hence, this is employed for the determination of phenyl vinyl sulfone in the eletriptan hydrobromide.

The objective of the proposed method is to develop and validate a specific, precise and accurate LC-MS method for the determination of 2-acetoxy ethyl acetoxy methyl ether (AEM) in Acyclovir. An isocratic separation was done by using YMC-PACK PRO C18, (150 x 4.6 mm, 3µm) column with a mobile phase composition of Buffer (0.1% Ammonia in water) : Methanol(25 :75v/v)at a flow rate of 0.4mL/min, diluent solution of 0.2% of 0.1N NaOH in Methanol were monitored on a triple quadrupole mass spectrometer, operating in the multiple reaction monitoring (MRM) Acquisition mode and possitive polarity mode. The Retention time of AEM was found to be 5.344 minutes, LOD and LOQ were observed at 0.03 ppm and 0.11 ppm concentration respectively by signal to noise ratio method, linearity was found in the concentration range of 0.18-0.55 ppm with correlation coefficient of 0.996 and accuracy in the range of 97.7- 101% was obtained by performing percentage recovery studies. The % RSD was observed to be less than 10% for six replicates said to be precise. The proposed method was validated for accuracy, precision, sensitivity, and linearity successfully employed for quantitative determination of AEM in acyclovir.

The main objective of the present research study is to develop and validate a sensitive, specific, accurate and precise LC-MS method for the determination of p-Chloroaniline and (S)-5-Chloro-α-(cyclopropylethynyl)-2- amino-α- (trifluoromethyl) benzene methanol in Efavirenz bulk form. The effective separation of p-Chloroaniline and (S)-5-Chloro-α-(cyclopropylethynyl)-2- amino-α- (trifluoromethyl) benzene methanol were achieved by using Hypersil BDS (C18, 100 x 4.6 mm, 3 µm) column and a solvent system of Buffer (0.1% Formic acid in water): Methanol (30:70 v/v) with a flow rate of 0.4 ml/min. The p-Chloroaniline and (S)-5-Chloro-α-(cyclopropylethynyl)-2- amino-α-(trifluoromethyl) benzene methanol were monitored on mass spectrometer coupled with atmospheric pressure chemical ionization, positive polarity mode and quadrapole mass analyzer. The Retention time of p-Chloroaniline, (S)-5-Chloro-α-(cyclopropylethynyl)-2- amino-α- (trifluoromethyl) benzene methanol and Efavirenz were found at 5.7min, 7.6min and 11.1min resepectively. The detection limit and quantification limit were observed at 0.25ppm and 0.75 ppm respectively for both p-Chloroaniline and (S)-5-Chloro-α-(cyclopropylethynyl)-2- amino-α-(trifluoromethyl) benzene methanol. Those analytes were linear in the concentration ranges from 0.75ppm to 3.75ppm and the percentage relative standard deviation of six replicates of same concentrations of both the analytes were less than 10%. Hence this method was effective in separation and determination of p-Chloroaniline and (S)-5-Chloro-α-(cyclopropylethynyl)-2- amino-α- (trifluoromethyl) benzene methanol in Efavirenz.