The aim of the study was to determine the nutritional value (content of endogenous and exogenous amino acids) and safety (content of metals and radionuclides) of the muscle tissue of fish obtained in the Shuchinsk- Burabay recreation area (Kazakhstan). Analytical methods involving mass spectrometry with inductively coupled plasma and liquid chromatography were used. The content of exogenous amino acids in fish protein was similar to their level in the meat of cattle, pigs, and poultry obtained in the same leisure zone. The ratio of the sum of exogenous amino acids to the sum of endogenous amino acids in fish meat protein ranged from 0.29 to 0.39. In all samples, it ranged from 45.45 to 33.33% of the norm. Samples of fish meat from some lakes diverged from the norm in the amount of exogenous and endogenous amino acids by 2.01-3%. Valine was a limiting amino acid in the meat of fish from all lakes; its amount ranged from 21.6 to 27.8%. The second most important limiting amino acid was isoleucine, which ranged from 72.1 to 77.25%. The contents of heavy metal salts were up to 0.0590 mg/kg for arsenic, 0.0050 mg/kg for cadmium, and 0.0350 mg/kg for lead, which did not exceed the norms of the maximum allowable concentration of these metals. Only the mercury content in perch muscle tissue, which was 0.0385 mg/kg, exceeded the maximum permissible concentration by 28%. The level of other metals was in line with the standards. Fish from the lakes of the Shuchinsk-Burabay recreation area are slightly contaminated with heavy metal salts, but these pollutants generally do not exceed the maximum permissible concentration. The concentration of radionuclides in fish samples did not exceed the maximum permissible level.
Aim: This study aimed to determine the food safety and protein adequacy of meats from various animals in the Shuchinsk- Burabay resort zone. Materials and Methods: Samples of meat were collected from markets "Zhomart" and "Kausar." Two hundred and ninety-eight samples of meat were obtained: beef - 166, horse - 42, pork - 67, mutton - 8, and poultry - 15. From each carcass, part of the carcass, point samples were taken to form an average sample and conduct research. Analyses used inductively coupled plasma mass spectrometry and liquid chromatography. The determination of amino acid composition was carried out following the chromatographic method for determining the amino acid composition of meat proteins. Results: The quality of meats from the resort zone was lower than recommended due to the reduced content of essential amino acids, including valine, lysine, isoleucine, and phenylalanine. Concentrations of heavy metals and radionuclides did not exceed maximum permissible limits, and trace concentrations of arsenic, cadmium, and lead were detected in all meat samples, except beef. The latter meat showed increased concentrations of mercury. Conclusion: In terms of quality indicators, all types of meat met standards; however, pork protein displayed the most favorable amino acid composition, both for content of essential amino acids and the ratio of amounts of essential to non-essential amino acids. For content of heavy metals, poultry and pork meats were safest.
The new methods for identification of honey adulteration is presented in the article. The method differs in reliability, speed and ease of application. There have been described the methods for evaluation of honey naturalness implemented in laboratories of veterinary and sanitary examination with their shortcomings. The method to identify honey adulteration has been developed, using the honey of different botanical origin (acacia, sunflower, rapeseed, fruticous, espartes, nectar flow, alpine, buckwheat, polyfloral). In total we used 72 samples of honey and conducted 162 laboratory researches. The diastatic number of honey was defined in accordance with All-Union Standard 19792-2001. The composition of artificial honey included sugar syrup and artificially inverted sugar
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