Introduction:The insertion of children in daycare center and pre-schools has been an important device in the current social configuration. However, t he p ermanent l iving i n the daycare center makes the children sickness more frequently when compared to others who remain in more restricted family spaces, especially for enteroparasites. Objectives: The present study aimed to evaluate the prevalence of Cryptosporidium spp. and Giardia spp., emerging and re-emerging opportunistic enteroparasites, and other enteroparasites in faeces samples from children aged 2 to 6 years old of a philanthropic daycare center. Patients and Methods: Thirty-two children participated in the study and the detection of enteroparasites was carried out by the method of Hoffmann, Pons and Janer. Identification o f Cryptosporidium s pp. w as c arried o ut by centrifugal-sedimentation formalin-ether followed by modified Ziehl-Neelsen staining. For confirmation of Cryptosporidium spp. and Giardia spp. the ELISA test (RIDASCREEN®) was used. Results: The prevalence of enteroparasites was 62.5%, with a high frequency of Cryptosporidium spp. (31%) and Giardia spp. (25%). The age most affected by these emerging and re-emerging microorganisms was 3 and 4 years old. Conclusion: From the above, health education processes for families and staff of scholar institutions on the main forms of transmission and prevention of enteroparasitoses are of paramount importance in order to avoid the spread of pathogenic microorganisms in the school environment. Descriptors:Communicable Diseases, Emerging; Cryptosporidium; Giardia; Child. Conflito de interesses:Não Contribuição dos autores: MGPFD, BMF, KAAT, SISM Concepção e planejamento do projeto de pesquisa; Obtenção ou análise/interpretação dos dados; Redação e revisão crítica. FCJ, CFB, CSM Obtenção ou análise/interpretação dos dados; Redação e revisão crítica. GPM, TVS, Redação e revisão crítica Contato para correspondência: Susana Inés Segura-Muñoz
High doses of antibiotics used in hospitals can affect the microbial composition of sewers, selecting resistant bacteria. In this sense, we evaluated the antibiotic resistance profile and the multiresistant phenotype of bacteria isolated in sewage from a tertiary hospital in the interior São Paulo state, Brazil. For bacteria isolation, 10 µL of sewage samples were sown in selective culture media and the isolates were identified using VITEK-2 automatized system. The antibiotic sensitivity test was performed by disk diffusion. High percentages of resistance were found for amoxicillin, ampicillin, ceftazidime, clindamycin, vancomycin and the multidrug-resistant phenotype (MDR) was attributed to 60.7% of the isolates. Our results show bacteria classified as critical/high priority by WHO List of Priority Pathogens (Enterococcus and Staphylococcus aureus resistant to vancomycin and Enterobacteriaceae resistant to carbapenems) in hospital sewage. Therefore, the implementation of disinfection technologies for hospital sewage would reduce the bacterial load in the sewage that will reach urban wastewater treatment plants, minimizing superficial water contamination and bacterial resistance spread in the environment.
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