No abstract

Emblica officinalis fruit extract has been incorporated into polymer nanofiber scaffold and the resulting scaffold showed excellent antibacterial and anti-proliferative properties.

Nano-sized materials have been an important tool in basic and applied sciences. A novel, low cost, green and reproducible bacteria, Staphylococcus aureus mediated biosynthesis of titanium dioxide nanoparticles (TiO2 NPs) was reported in the present study. Initial conformational studies were done using UV-visible spectroscopy and confirmed the synthesis of TiO2 NPs in the broth. The detailed characterization of the TiO2NPs was carried out using SEM, XRD, FTIR and Raman spectroscopy. From the SEM, it was confirmed that the sample showed the NPs were smooth and spherical with an average diameter of about 20nm. From FTIR analysis, it was confirmed that the TiO2 nanoparticles are crystalline in nature, which was confirmed by the FTIR peak at 518cm-1 corresponds to the TiO2 vibration present in a crystalline structure. Additionally, the synthesized NPs were also characterized by Transmission Electron Microscopy (TEM) and Particle size analyzer. This study was aimed to determine the antibacterial and antibiofilm activity of Titanium oxide nanoparticles against both Gram-positive and Gram-negative bacterial species and significant positive results against Bacillus subtilis and Escherichia coli were observed.

In the bone tissue engineering composite scaffolds with osteogenic potential are emerging as the new tool. Here, we investigated the graphene (GP), graphene oxide (GO) andCissusquadrangularis(CQ) callus extract for their spontaneous osteoinductive potential. Electrospun poly ε-caprolactone (PCL) sheets were painted with varying combination GP, GO and CQ solutions as ink. The prepared PCL-GO, PCL-GO-CQ, PCL-GP and PCL-GP-CQ scaffolds were characterized for their physical, mechanical and biological properties. Addition of GO, GP, GO-CQ and GP-CQ to PCL enhanced roughness, wettability, Yield strength and tensile strength, biocompatibility .significantly. Presence of GO and CQ in PCL-GO-CQ scaffolds, while GP and CQ in PCL-GP-CQ scaffolds showed synergistic effect on the biocompatibility, Cell attachment,cell proliferation of human umbilical Wharton’s jelly derived mesenchymal stem cells (hUCMSCs) and their differentiation into osteoblasts by 21stday in culture without osteogenic differentiation media or any growth factors. Same is confirmed by the Alizarin red S staining and Von kossa staining. The combination of PCL-GO-CQ scaffold prepared by novel paint method was found to be the most potential in bone tissue engineering.