Ginger is an important monocotyledonous plant belonging to the family Zingiberaceae. The objective of this study was to investigate the regeneration potential of ginger using leaf base explants. Auxins such as 2, 4-D and NAA in combination with BA were used for initiation of callus. Different combinations of both ammonium (NH 4+) and nitrate (NO 3-) were also studied for efficient callus production. High frequency of white friable calli was observed on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2, 4-D, 0.5 mg/L NAA and 0.5 mg/L BA. The highest shoot induction (92.33%), shootlets number (7.33± 0.33) and length (88.33±4.40) mm were achieved on MS media containing 0.5 mg/L BA. Regenerated shoots were transferred to in vitro rooting media containing 1.0 mg/L IBA. Afterwards, plantlets with well-developed root and shoot system were subjected to a twostep hardening process. 71% of plantlets survived after secondary hardening without any abnormal morphology.

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An improved in vitro mass propagation protocol was developed for Aerva lanata using MS liquid medium. The influence of MS medium (solid and liquid) with cytokinin (TDZ and BAP, respectively) were studied for shoot proliferation and growth. The liquid medium perfomed better than solid medium in shoot multiplication. The maximum shoot multiplication rate was (29.37 ± 0.64 shoots per explant), obtained in MS liquid medium which is containing 0.6 mg/l TDZ, 0.3 mg/l NAA and 0.2 mg/l IBA. Different volumes of liquid medium have been used, 30 ml of medium flask showed the maximum number of shoots. Liquid medium is better suited for in vitro propagation of A. lanata since the enhanced multiplication rate was observed with shorter subculture intervals. Plant Tissue Cult. & Biotech. 31(1): 35-42, 2021 (June)

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