Biases in adult sex ratios can alter the intensity of sexual selection by enhancing competition for mates. Under intense competition males increase their investment in behaviors to outcompete rivals (e.g., fighting). Yet, given that in male-biased environments mating opportunities are rare males may alternatively reduce costly courtship and/or adopt alternative reproductive tactics (ARTs). Males of the spider Pisaura mirabilis adopt different mating tactics, offering females genuine nuptial gifts (prey), nutritionally worthless gifts (prey leftovers), or no gifts. To test whether behavioral shifts between gift tactics are triggered by changes in the competitive environment, we established replicate spider populations under natural conditions at varying adult sex ratios (male-biased, female-biased and equal) and sampled gift tactics repeatedly over time. We additionally explored how male individual traits, such as body size and condition, affect the expression of ARTs. In male-biased populations males produced more gifts but of low quality, suggesting competition to trigger increased mating effort to ensure mate acquisition and fertilizations, but through a worthless gift tactic. Production of gifts and of genuine gifts was favored by high body condition, pointing to energetic limitations as being central for male reproductive capacity. We hence highlight two co-existing mechanisms at play to explain ARTs in this system, the competitive social environment where expression of gift tactics is based on optimal-decision making to overcome competition, and a conditional strategy linked to the individual’s energetic state.

The Manila clam (Ruditapes philippinarum) is the second most exploited bivalve in the world but remains threatened by diseases and global changes. Their associated microbiota play a key role in their tness and acclimation capacities. This study aimed at better understanding the behavior of clam digestive glands and extrapallial uids microbiota at small, but contrasting spatial and temporal scales. Results showed that environmental variations impacted clam microbiota differently according to the considered tissue. Each clam tissue presented its own microbiota, and showed different dynamics according to the intertidal position and sampling period. Extrapallial uids microbiota was modi ed more rapidly than digestive glands microbiota, for clams placed on the upper and lower intertidal position, respectively.Clam tissues could be considered as different microhabitats for bacteria as they presented different responses to small-scale temporal and spatial variabilities in natural conditions. These differences underlined a more stringent environmental lter capacity of the digestive glands.

The Manila clam (Ruditapes philippinarum) is the second most exploited bivalve in the world but remains threatened by diseases and global changes. Their associated microbiota play a key role in their fitness and acclimation capacities. This study aimed at better understanding the behavior of clam digestive glands and extrapallial fluids microbiota at small, but contrasting spatial and temporal scales. Results showed that environmental variations impacted clam microbiota differently according to the considered tissue. Each clam tissue presented its own microbiota, and showed different dynamics according to the intertidal position and sampling period. Extrapallial fluids microbiota was modified more rapidly than digestive glands microbiota, for clams placed on the upper and lower intertidal position, respectively. Clam tissues could be considered as different microhabitats for bacteria as they presented different responses to small-scale temporal and spatial variabilities in natural conditions. These differences underlined a more stringent environmental filter capacity of the digestive glands.