Microbiota of the Digestive Glands and Extrapallial Fluids of Clams Evolve Differently Over Time Depending on the Intertidal Position

Offret, Clément; Gauthier, Olivier; Despréaux, Garance; Bidault, Adeline; Corporeau, Charlotte; Miner, Philippe; Petton, Bruno; Fabioux, Caroline; Paillard, Christine; Blay, Gwenaëlle Le

doi:10.1007/s00248-022-01959-0

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…There is also indirect evidence for the influence of temporal variation on bacterial communities: acylhomoserine lactones (AHLs), which are key bacterial signals within biofilms that are degraded by pH-and temperature-dependent hydrolysis, vary seasonally in concentration, being higher in winter and lower in summer (Roggatz and Parsons, 2022). The intertidal filter-feeding clam (Ruditapes philippinarum) is closely related to the microorganisms in its environment, and its extrapallial fluids show strong temporal variation in bacterial alpha-diversity between months (Offret et al, 2023).…”

Section: Temporal Changes In Intertidal Sediment Biofilmsmentioning

confidence: 99%

Spatio-temporal variation of bacterial community structure in two intertidal sediment types of Jiaozhou Bay

Chen¹,

Zhang²,

Huang³

et al. 2023

Preprint

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The intertidal sediment environment is dynamic and the biofilm bacterial community within it must constantly adjust, but an understanding of the differences in the biofilm bacterial community within sediments of different types is still relatively limited. In this study, the structure of the bacterial community in Jiaozhou Bay sediment biofilms are described using high-throughput 16S rRNA gene sequencing and the effects of temporal change and different sediment environment types are discussed. The Shannon index was significantly higher in sandy samples than in muddy samples. The co-occurrence network was tighter and more species were involved in community building in sandy samples. The principal coordinates analysis identified a significant separation between different sediment types and between stations (LiCun estuary, LC and ZhanQiao Pier, ZQ). Proteobacteria, which had a relative abundance of approximately 50% at all phylum levels, was significantly more abundant at ZQ, while Campilobacterota and Firmicutes were significantly more abundant at LC. The relative abundances of Bacteroidetes, Campilobacterota, Firmicutes, and Chloroflexi were significantly higher in the muddy samples, while Actinobacteria and Proteobacteria were higher in the sandy samples. There were different phylum-level biomarkers between sediment types at different stations. There were also different patterns of functional enrichment in biogeochemical cycles between sediment types and stations with the former having more gene families that differed significantly, highlighting their greater role in determining bacterial function. The RDA results, where each month’s samples were concentrated individually, showed reduced variation between months when the amplicon sequence variant was replaced by KEGG orthologs, presumably the temporal change had an impact on shaping the intertidal sediment bacterial community, although this was less clear at the gene family level. Random forest prediction yielded a combination of 43 family-level features that responded well to temporal change, reflecting the influence of temporal change on sediment biofilm bacteria.HighlightsSandy sediments have more bacterial species involved in community building.Different substrates from different stations have their own phylum biomarkers.Substrates have a greater influence on shaping bacterial function.Temporal changes have a greater shaping power on bacteria than on gene families.Graphic abstract

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Section: Temporal Changes In Intertidal Sediment Biofilmsmentioning

confidence: 99%

Spatio-temporal variation of bacterial community structure in two intertidal sediment types of Jiaozhou Bay

Chen¹,

Zhang²,

Huang³

et al. 2023

Preprint

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“…This is actually observed for a fair proportion of their microbiota, which is rapidly eliminated upon depuration with purified seawater, a common method employed for removing waterborne microbial contaminants from bivalves [ 14 ]. Still, the microbiota of bivalves is highly distinct from the microbial communities found in the surrounding seawater [ 8 , 9 , 14 – 16 ]. Moreover, among bivalve-associated bacteria, some genera, which share the same habitat and may have a long co-evolutionary history with their hosts, are little affected by environmental variations [ 14 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Cross-talk and mutual shaping between the immune system and the microbiota during an oyster's life

Destoumieux-Garzón,

Montagnani,

Dantan

et al. 2024

Phil. Trans. R. Soc. B

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The Pacific oyster Crassostrea gigas lives in microbe-rich marine coastal systems subjected to rapid environmental changes. It harbours a diversified and fluctuating microbiota that cohabits with immune cells expressing a diversified immune gene repertoire. In the early stages of oyster development, just after fertilization, the microbiota plays a key role in educating the immune system. Exposure to a rich microbial environment at the larval stage leads to an increase in immune competence throughout the life of the oyster, conferring a better protection against pathogenic infections at later juvenile/adult stages. This beneficial effect, which is intergenerational, is associated with epigenetic remodelling. At juvenile stages, the educated immune system participates in the control of the homeostasis. In particular, the microbiota is fine-tuned by oyster antimicrobial peptides acting through specific and synergistic effects. However, this balance is fragile, as illustrated by the Pacific Oyster Mortality Syndrome, a disease causing mass mortalities in oysters worldwide. In this disease, the weakening of oyster immune defences by OsHV-1 µVar virus induces a dysbiosis leading to fatal sepsis. This review illustrates the continuous interaction between the highly diversified oyster immune system and its dynamic microbiota throughout its life, and the importance of this cross-talk for oyster health. This article is part of the theme issue ‘Sculpting the microbiome: how host factors determine and respond to microbial colonization’.

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“…Host-microbiota relationships are of broad interest to researchers in marine invertebrates, and hosts can establish microbiota associated with healthy homeostasis through vertical and lateral transmission [3]. Tese host microorganisms signifcantly impact the host's digestive function, metabolism, and infection immunity [4,5], as well as assist the host in adapting to a changing environment and acting as a barrier against pathogens. Microbial composition structures are known to serve as health status markers in bivalve shellfsh, and potential pathogens may lead to potential disease outbreaks during ecological dysregulation [3,6].…”

Section: Introductionmentioning

confidence: 99%

Effects of Natural and Artificial Seawater on Digestive Gland and Gill Microorganisms of the Manila Clam (Ruditapes philippinarum) during Temporary Rearing without Feeding

Zhu

Zhang

Liu

et al. 2023

Aquaculture Research

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The bacteria of shellfish bioaccumulate mainly in the gill and digestive gland tissues, which can affect shellfish health status and disease susceptibility under stressful conditions or environmental effects. Ruditapes philippinarum is a filter-feeding shellfish with both ecological and economic significance, and it is classified as a Mollusca (phylum), Bivalvia (class), Veneridae (family), and Ruditapes (genus). In this study, 16S high-throughput sequencing was used to explore the microbiomics of digestive glands and gills of R. philippinarum under temporary rearing without feeding (including the purification process) in different water environments (natural vs. artificial seawater) from 0–7 days. The results revealed that the digestive glands and gills of R. philippinarum had their own unique bacterial community structures. Tissue microorganisms under the overlap of different water environments and starvation factors showed different dynamic effects within 0–7 days. The sand spitting and purification steps (posttransport rehydration stage of 24 hours) effectively reduced microorganism abundance. There were different biomarkers in the prerearing and postrearing periods, and R. philippinarum may be more susceptible to the enrichment of opportunistic pathogenic bacteria during the postrearing period in artificial seawater.

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Microbiota of the Digestive Glands and Extrapallial Fluids of Clams Evolve Differently Over Time Depending on the Intertidal Position

Cited by 6 publications

References 42 publications

Spatio-temporal variation of bacterial community structure in two intertidal sediment types of Jiaozhou Bay

Spatio-temporal variation of bacterial community structure in two intertidal sediment types of Jiaozhou Bay

Cross-talk and mutual shaping between the immune system and the microbiota during an oyster's life

Effects of Natural and Artificial Seawater on Digestive Gland and Gill Microorganisms of the Manila Clam (Ruditapes philippinarum) during Temporary Rearing without Feeding

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