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HighlightsCabbage “nutrient rich” economically important nutrient rich vegetable crop with secondary metabolites called glucosinolates contributed to anticarcinogenic properties.Various stress (biotic and abiotic) results in quantitative and qualitative yield losses. So application of plant genetic engineering techniques can be used to cope up with the stresses.The goal of present investigation was to develop an efficient, reliable and high-frequency plant-regeneration protocol for the introduction of a desirable gene in cabbage, was successfully achieved.

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The importance of germplasm characterization is an important link between the conservation and utilization of plant genetic resources in various breeding programmes. In the present study, genetic variability and relationships among 25 L. genotypes were tested using random amplified polymorphic DNA (RAPD) molecular markers. A total of 45 random decamer oligonucleotide primers were examined to generate RAPD profiles, out of these reproducible patterns were obtained with 22 primers. A total of 87 amplicon were obtained, out of which all were polymorphic and 7 were unique bands. The level of polymorphism across genotypes was 100% as revealed by RAPD. Genetic similarity matrix, based on Jaccard's coefficients ranged from 13.7 to 84.10% indicating a wide genetic base. Dendrogram was constructed by unweighted pair group method with arithmetic averages method. RAPD technology could be useful for identification of different accessions as well as assessing the genetic similarity among different genotypes of lettuce. The study reveals the limited genetic base and the needs to diversify using new sources from the germplasm.

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