This paper presents the integration of micropaleontological (palynology and foraminifera) and isotopic ( 87 Sr/ 86 Sr) analysis of a selected interval from the well 2-TG-96-RS, drilled on the onshore portion of the Pelotas Basin, Rio Grande do Sul, Brazil. A total of eight samples of the section between 140.20 and 73.50 m in depth was selected for palynological analysis, revealing diversified and abundant palynomorph associations. Species of spores, pollen grains and dinoflagellate cysts are the most common palynomorphs found. Planktic and benthic calcareous foraminifera were recovered from the lowest two levels of the section (140.20 and 134.30 m). Based on the stratigraphic range of the species of dinoflagellate cysts and sporomorphs, a span age from Late Miocene to Early Pliocene is assigned. The relative age obtained from the 87 Sr/ 86 Sr ratio in shells of calcareous foraminifers indicates a Late Miocene (Messinian) correspondence, corroborating the biostratigraphic positioning performed with palynomorphs. Paleoenvironmental interpretations based on the quantitative distribution of organic components (palynomorphs, phytoclasts and amorphous organic matter) throughout the section and on foraminiferal associations indicate a shallow marine depositional environment for the section. Two palynologicals intervals were recognized based on palynofacies analysis, related to middle to outer shelf (140.20 to 128.90 m) and inner shelf (115.75 to 73.50 m) conditions.

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After the formalization of the base of the Miocene in the Lemme‐Carrosio section (Italy) at the base of Subchron C6Cn.2n, the calcareous plankton biostratigraphy was refined in several open ocean Deep Sea Drilling Project/Ocean Drilling Program sites. However, high‐resolution quantitative biostratigraphic studies, integrating planktonic foraminifera and calcareous nannofossils, are still lacking for the time interval spanning the Oligocene–Miocene transition. Here, we present a reinvestigation of Deep Sea Drilling Project Hole 516F (Rio Grande Rise) and 4 oil wells drilled by Petróbras Brasileiro SA in the Campos Basin (SW Atlantic Ocean). We identified 12 planktonic foraminiferal and 18 calcareous nannofossil bioevents that have been integrated with an updated magnetostratigraphy of Hole 516F allowing the correlation with the GPTS and the identification of the Oligocene/Miocene boundary (base of Subchron C6Cn.2n) between the Top of Sphenolithus delphix and the Base of common Paragloborotalia kugleri. Furthermore, our results give new insights on the reliability of major calcareous plankton events across the Oligocene–Miocene transition: (a) the Sphenolithus ciperoensis Top, the S. delphix Base and Top, and the Sphenolithus cometa Base are reliable events at a global scale; (b) the Bases of Globoquadrina dehiscens and Sphenolithus disbelemnos > 4 μm are correlatable events only within the study sector of the SW Atlantic Ocean; and (c) the Globoturborotalita ciperoensis Top, Globoturborotalita angulisuturalis Top, and Sphenolithus procerus Base are diachronous. Finally, previously unreported biostratigraphic data, such as the distribution range of S. disbelemnos < 4 μm and Sphenolithus cf. S. pseudoheteromorphus, the Tenuitellinata praestainforthi acme interval, and the Top of common Globigerinoides primordius were identified in the Campos Basin.