No abstract

Three Gram-reaction-positive bacteria, isolated from the air in a medical practice (strains WS4601 T , WS4602) or a pharmaceutical clean room (strain WS4599), were characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA and recA gene sequences of the three novel strains showed that they formed a distinct lineage within the genus Micrococcus, sharing 16S rRNA gene sequence similarities of 96.1-98.0 % with other species of this genus. Chemotaxonomic features also supported the classification of the three novel strains within the genus Micrococcus. The major cellular fatty acids of strain WS4601 T were anteiso-C 15 : 0 and iso-C 15 : 0 , the cell-wall peptidoglycan was of type A3a (L-Lys-L-Ala), and the predominant respiratory quinones were MK-7(H 2 ) and MK-8(H 2 ). The polar lipid profile contained diphosphatidylglycerol and phosphatidylglycerol, but no phosphatidylinositol. The G+C content of the genomic DNA was 70.4 mol%. Numerous physiological properties were found that clearly distinguished strains WS4599, WS4601 T and WS4602 from established members of the genus

Four Gram-stain-positive, aerobic bacterial strains isolated from a pharmaceutical clean room (strain WS4616T), a dessert milk product (strain WS4617) and from raw milk (strains WS4623 and WS4624) were characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA and recA gene sequences showed that they formed a distinct lineage within the family Propionibacteriaceae . Similarity values between 16S rRNA gene sequences of the four novel strains and the type species of all genera belonging to the family Propionibacteriaceae were 89.2–94.1 %. The major cellular fatty acid was anteiso-C15 : 0 and the major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. Respiratory quinones were MK-8(H4) and MK-9(H4). The cell-wall peptidoglycan of type A3γ contained ll-diaminopimelic acid, alanine, glycine and glutamic acid. The G+C content of the genomic DNA of strain WS4616T was 67.7 mol%. The whole-cell sugar pattern contained ribose, mannose, arabinose, glucose and galactose. On the basis of phenotypic and genetic data, strains WS4616T, WS4617, WS4623 and WS4624 are classified as members of a novel species in a new genus of the family Propionibacteriaceae , for which the name Naumannella halotolerans gen. nov., sp. nov. is proposed. The type strain is WS4616T ( = DSM 24323T = LMG 26184T) and three additional strains are WS4617, WS4623 and WS4624.