Recurrent miscarriage (RM) is defined as the loss of pregnancy three or more consecutive times in the first and second trimester, which in some cases occurs due to immune abnormalities. This study aimed to assess some immunological parameters in women with recurrent miscarriages, including the level of antiphospholipid antibody (APA), anticardiolipin (ACA), antinuclear antibody (ANA), complement C3 and C4, and interleukine-3 (IL-3). We included 100 patients together with 100 healthy women as a control. ELIZA was used to measure some types of autoantibodies. APA and ACA significantly increased (P≤0.05) in patients compared to control. In addition, 29% of the patients were positive for antinuclear antibodies (ANA), while the control subjects had negative results for these autoantibodies. Regarding the complement, the serum levels of C3 and C4 were significantly elevated in the serum level of patients when compared to the control group, but in treated patients (heparin and low-dose aspirin), the levels of the complement (C3 and C4) showed a significant decrease in patients compared to total controls. Cytokine level (IL-3) significantly decreased in untreated patients 302.78 pg/ml compared to treated patients (741.57 pg/ml). Antiphospholipid antibodies are more prevalent among women with recurrent miscarriages and are also believed to be the result of abnormal autoimmune activation.

Immune markers are enzymes which influence human capacity to fight off dangerous agents like germs as well as various external invaders. This paper aims to determine the elements that have a role in the pathogenesis of Thyroid disease, an immunological disorder caused by a combination of hereditary predisposition and external conditions. Celiac disease (CD), also known as gluten sensitive enteropathy, is rather prevalent in western countries, with just a frequency of roughly 1%. Most people that are asymptomatic or have minor signs may now be diagnosed with CD because to the new advent of precise or precise serological tests. With this using vitro humanoid creature, investigate overall effects of hypothyroidism function in circulatory biomarkers for fibroblast immunological reaction. This pathophysiology of autoimmune thyroid disorders (AITD) is inadequately known, as well as the relationship among immunological characteristics with AITD-related genetic variations is still unknown. Individuals' thyroid functioning progressed from asymptomatic or moderate hyperthyroidism at the first appointment to typical circulation amounts of unbound thyroid hormone or thyroid - stimulating hormone just at subsequent, eventually leading to hypothyroidism. Researchers recently explored that anti-thyroid peroxidase antibody (TPOAb) concentrations were connected with global reduction in IgG central fucosylation and antennary fucosylation in periphery plasma mononuclear cells in AITD. Fucose reduction is known to enhance targeted antigen-expressing cell killing by potentiating robust antibiotic Killer cellular proliferation. It might lead to autoantibody-mediated immune cell mobilization or assault of self-antigen-expressing normal tissues in autoimmunity.

SLE (Systemic Lupus Erythematosus)  is, a heterogeneous disease, with ,diverse clinical manifestation disorder characterized by hyperactivity of  B and T cell, creation  of auto, antibodies, and, deposition, of antibody- containing immune complexes in blood vessels, during body. This study aims to investigation some immunological markers related to patients with SLE. It includes the study of the level, of Interleukin, (IL.17), antinuclear antibody (ANA), anti dsDNA, antiphospholipid and, complement, C3, C4, In addition to study the complete blood picture including hemoglobin, red blood cell monocytes, white blood cells, platelets and lymphocytes, they belong to factors that may interfere with the disease.  Current study included 110 patients with SLE, and 106 (96.36%) were female and 4 (3.64%) were male. Patients with an average age of 31.61 ± 8.60 years. This work also includes 70 blood specimens as control group. Our study illustrated that most infections were on Village 71.82 % compared with the City 28.18 , and patients without history disease  57.27 % while patients with history disease42.73%.    The results also showed that the concentration of IL.17 was significantly higher than p ≤ 0.05 in serum patients with lupus erythematosus (469 ± 2.25 g / ml)  comparison with healthy individuals (107.39 ± 0.41 pg / ml).  ELISA was used to measure some types of autoantibodies, and showed an increase in ANA and dsDNA Ab levels in SLE patients compared with healthy individuals. The results also prone that the antiphospholipid is significantly increase (P ≤ 0.05) in patients with SLE compared to controls.Evaluating serum complement  level C3, C4 in the total study, our results observed  significant decrease (p ≤ 0.05) in serum of patients with SLE (0.62 ± 0.01,0.13 ± 0.01g/l) respectively, compared with controls ,(1.74 ± 0.01,0.28 ± 0.01g/l) respectively.The results of the study also showed a significant decrease in hemoglobin, red blood cell monocytes, white blood cells, platelets and the number of lymphocytes for SLE patients compared with healthy individuals.The relationship between antibodies to ANA and IL-17, antibodies to dsDNA and C3, C4 and lymphocytes was identified, as well as the relationship between APA, C3 and C4 antibodies.    Inconclusion  ,peoples with SLE have a positive ANA, High  concentration of, ds DNA Ab and anti-phospholipid which plays an important role in pathogenesis and complement C3 and C4 in patients with SLE is decreased. An increased inflammatory concentration of interleukin (IL.17) can play a critical role in the pathogenesis of SLE which leads to disease progression.

The aim: The aim of this research is to evaluate some immunological biomarkers in cases of Rheumatoid arthritis and to verify their correlation with activity of disease among the population of Thi-Qar province. Matherials and methods: This study included 45 cases of rheumatoid arthritis and 45 healthy subjects. All cases underwent complete history taking, thor¬ough clinical examination, and laboratory tests including erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), Anti-citrulline antibody (Anti-CCP) and rheumatoid factor (RF). IL-17and TNF-α blood level was measured by Enzyme Linked Immunosorbent Assay (ELISA) method. DAS-28 (Disease activity score 28) was evaluated. Results: Serum levels TNF-α was higher in Rheumatoid arthritis patients (424.3±19.46 pg/ml) than in healthy individuals (112.7±4.73 pg/ml), and IL-17 blood levels were higher in Rheumatoid arthritis patients (233.5±241.4 pg/ml) than the healthy individuals group (47.24±49.7 pg/ml). There was significant association found among IL-17, DAS-28, CRP and hemoglobin levels. Conclusions: In conclusion, IL-17 blood levels were significantly increased in peoples with rheumatoid arthritis than in healthy individuals. Its significant relationship with DAS-28 suggested that the level of IL-17 in serum could be important immunological biomarker for activity of disease in disease of Rheumatoid arthritis.

Type 1 diabetes mellitus (T1D) is a chronic autoimmune illness defined as insulin insufficiency resulting from the autoimmune breakdown of pancreatic beta cells producing insulin in the islets of Langerhans. Biomarkers are markers of physiological or pathological processes that are normal or abnormal, playing a crucial function in clinical evaluation, prognosis, and therapy response monitoring. This study aimed to investigate some biomarkers associated with T1D and examine the association between glutamic acid carboxylase (GADA) antibody and islet antigen-2 autoantibody (IA-2A) for β-cell stress and death in patients with T1D. The current study included 60 patients with T1D, 32 (53.33%) males and 28 (46.67%) females between 9 to 18 years old, and 30 healthy individuals as control. Glutamic acid carboxylase, islet antigen-2 autoantibody and connecting peptide levels in the blood were evaluated. Positive results for IA-2A and GADA were shown in 89.04% and 38% of T1D patients, respectively. The normal level frequency and C-peptide titer mean were significantly lower between T1D and healthy control. However, no statistically significant changes were observed in the C-peptide level among GADA positive and negative patients. Finally, the C-peptide concentrations were significantly lower for positive IA-2A compared to negative IA-2A persons. The combination of IA-2A, GADA, and C-peptide could indicate stronger diagnostic measures at a low cost for patients with T1D.