Enhanced nodulation in cluster root zones and increased potential for organic acid production in root nodules appear to contribute to white lupin's resilience to P-deficiency.
The major opportunities for broader incorporation of bioinformatics in education can be placed into three general categories: general applicability of bioinformatics in life science and related curricula; inherent fit of bioinformatics for promoting student learning in most biology programs; and the general experience and associated comfort students have with computers and technology. Conversely, the major challenges for broader incorporation of bioinformatics in education can be placed into three general categories: required infrastructure and logistics; instructor knowledge of bioinformatics and continuing education; and the breadth of bioinformatics, and the diversity of students and educational objectives. Broader incorporation of bioinformatics at all education levels requires overcoming the challenges to using transformative computer-requiring learning activities, assisting faculty in collecting assessment data on mastery of student learning outcomes, as well as creating more faculty development opportunities that span diverse skill levels, with an emphasis placed on providing resource materials that are kept up-to-date as the field and tools change.
This project outlines an 8‐week undergraduate laboratory which investigates the use of siRNA technology to silence a well‐known housekeeping gene, Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) in a mammalian HeLa cell line. siRNA was discovered just over ten years ago, however there are few published reports on the use of this important technology in the undergraduate curriculum. This laboratory for upper level science majors, aimed to improve students’ mastery of modern day experimental techniques and strengthen their molecular biology lab skills by working with RNA, an inherently unstable molecule. Presented student data will showcase their successful efforts to knockdown GAPDH using siRNA through fluorescence, Western Blot and GAPDH assays. In addition to the experimental results, our course embedded assessment will show that students gained significant confidence in understanding and applying siRNA technology. This project was supported by a NSF CCLI grant #068183789.
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